11388908910

Biotin-16-UTP

pkg of 25 μL (250 nmol; 10mM)

• Synonym(s): Biotin-16-UTP, biotin
• UNSPSC Code: 41116100
• NACRES: NA.54
• Assay: 97.7% (HPLC)
• Form: solution
• Solubility: water: miscible
• Storage temp.: −20°C

Properties

• assay: 97.7% (HPLC)
• form: solution
• mol wt: M_r 987.5 (biotin-16-UTP-Li₄)
• packaging: pkg of 25 μL (250 nmol; 10mM)
• manufacturer/tradename: Roche
• color: colorless
• solubility: water: miscible
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling.[1] Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.

Application

Biotin-labeled RNA can be used as a hybridization probe for:

• Northern blots
• Southern blots
• Plaque or colony lifts
• RNase protection experiments
• In situ hybridization
• Microarray hybridization[2][3]

The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.

Physical form

Biotin-16-UTP, tetralithium salt, 10mM solution

Analysis Note

Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash
• Storage Class: 12 - Non Combustible Liquids