11388908910
Roche
Biotin-16-UTP
pkg of 25 μL (250 nmol; 10mM)
Synonym(s):
Biotin-16-UTP, biotin
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25 μL
$268.00
$268.00
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25 μL
$268.00
About This Item
UNSPSC Code:
41116100
$268.00
In StockDetails
Recommended Products
assay
97.7% (HPLC)
Quality Level
form
solution
mol wt
Mr 987.5 (biotin-16-UTP-Li4)
packaging
pkg of 25 μL (250 nmol; 10mM)
manufacturer/tradename
Roche
color
colorless
solubility
water: miscible
shipped in
dry ice
storage temp.
−20°C
1 of 4
This Item | 11093070910 | 11427598910 | 11685597910 |
|---|---|---|---|
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche |
| assay 97.7% (HPLC) | assay - | assay ≥98% (HPLC) | assay - |
| form solution | form solution | form solution | form solution |
| shipped in dry ice | shipped in - | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling.[1] Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.
Application
Biotin-labeled RNA can be used as a hybridization probe for:
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
Quality
Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).
Physical form
Biotin-16-UTP, tetralithium salt, 10mM solution
Other Notes
For life science research only. Not for use in diagnostic procedures.
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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