- 11412272001
11412272001
Roche
Transforming Growth Factor-β1, human (hTGF-β1)
recombinant (CHO cells)
Recommended Products
biological source
human
Quality Level
recombinant
expressed in CHO cells
Assay
>95% (SDS-PAGE)
form
solution
potency
<0.1 ng/mL EC50
mol wt
25,000 Da
packaging
pkg of 1 mL (1 μg)
manufacturer/tradename
Roche
storage condition
avoid repeated freeze/thaw cycles
impurities
<0.1 EU/μgtested (LAL test)
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... TGFB1(7040)
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This Item | T2815 | H8666 | T7039 |
|---|---|---|---|
| biological source human | biological source human | biological source human | biological source human |
| recombinant expressed in CHO cells | recombinant expressed in NSO cells | recombinant expressed in HEK 293 cells | recombinant expressed in CHO cells |
| assay >95% (SDS-PAGE) | assay ≥97% (electrophoresis) | assay ≥95% (SDS-PAGE) | assay ≥98% (SDS-PAGE) |
| form solution | form powder | form lyophilized powder | form powder |
| potency <0.1 ng/mL EC50 | potency 0.04 ng/mL ED50 | potency ≤0.5 ng/mL EC50 | potency ≤0.05 ng/mL ED50 |
| mol wt 25,000 Da | mol wt 25 kDa | mol wt dimer 25 kDa (non-glycosylated) | mol wt protein 25 kDa |
General description
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.
Specificity
Specific activity/EC50: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.
Application
Biochem/physiol Actions
Quality
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)
Sequence
Unit Definition
Preparation Note
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Other Notes
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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