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11412272001

Roche

Transforming Growth Factor-β1, human (hTGF-β1)

recombinant (CHO cells)

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Synonym(s):
hTGF
UNSPSC Code:
12352207

biological source

human

Quality Level

recombinant

expressed in CHO cells

assay

>95% (SDS-PAGE)

form

solution

potency

<0.1 ng/mL EC50

mol wt

25,000 Da

packaging

pkg of 1 mL (1 μg)

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

impurities

<0.1 EU/μgtested (LAL test)

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... TGFB1(7040)

General description

Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.

Specificity

Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.
Specific activity/EC50: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.

Application

Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.

Biochem/physiol Actions

Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells.
Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.

Quality

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)

Sequence

Chain Length 2 x 112 AA
Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.

Unit Definition

EC50 definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.

Preparation Note

Working concentration: 0.01 - 10 ng/ml
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Other Notes

For life science research only. Not for use in diagnostic procedures.

wgk_germany

nwg

flash_point_f

does not flash

flash_point_c

does not flash


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Periostin is essential for periodontal ligament remodeling during orthodontic treatment.
Xu HY, et al.
Molecular Medicine Reports, 15(4), 1800-1806 (2017)
α-MSH stimulation contributes to TGF-β1 production via MC1R-MITF signaling pathway in melanoma cell
Hayashi E, et al.
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Cloning, expression, and characterization of chicken transforming growth factor ?4
Pan, Hongjie, and Jaroslava Halper
Biochemical and Biophysical Research Communications, 24-30 (2003)
Barbara Chiavarina et al.
Theranostics, 11(4), 1626-1640 (2021-01-08)
Colorectal cancer (CRC) cells are traditionally considered unresponsive to TGFβ due to mutations in the receptors and/or downstream signaling molecules. TGFβ influences CRC cells only indirectly via stromal cells, such as cancer-associated fibroblasts. However, CRC cell ability to directly respond

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