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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 5 reactions | Available to ship TODAYfromMILWAUKEE | $466.00 |
About This Item
UNSPSC Code:
12352200
NACRES:
NA.54
Technique(s):
ELISA: suitable, immunoblotting: suitable
Usage:
sufficient for 5 labeling reactions
Storage temp.:
2-8°C
$466.00
Available to ship TODAYDetails
usage
sufficient for 5 labeling reactions
packaging
kit of 1 (5 components)
manufacturer/tradename
Roche
technique(s)
ELISA: suitable, immunoblotting: suitable
storage temp.
2-8°C
General description
For labeling of proteins with biotin.
Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.
Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.
Application
Biotin protein labeling kit has been used to produce biotinylated denatured bovine serum albumin (d-BSA) for coating cadmium telluride (CdTe) quantum dot (QD) conjugates.
The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.[1]
The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.[1]
Packaging
1 kit containing 5 components.
Preparation Note
Working solution: Preparation of the solutions
- Blocking solution: Dissolve the contents of the bottle blocking reagent (green screw cap) in 300 ml double-distilled water.
- PBS solution: Dissolve the contents of the bottle PBS (blue screw cap) in 1 I double-distilled water.
- Biotin-7-NHS solution: The solution should be freshly prepared before labeling. Add to the contents of the reaction vial which includes biotin-7-NHS 250 μI DMSO and vortex the solution several times. The resulting concentration of the biotin-7-NHS solution is 20 mg/ml. For the labeling mix, the volume of the biotin-7-NHS solution should not exceed 5% of the total reaction volume (e.g., add maximal 50 μl biotin-7-NHS solution to 1 ml protein solution).
Analysis Note
Function tested.
Other Notes
For life science research only. Not for use in diagnostic procedures.
1 of 1
This Item | |||
|---|---|---|---|
| usage sufficient for 5 labeling reactions | usage sufficient for 50 labeling reactions | usage - | usage - |
| technique(s) ELISA: suitable, immunoblotting: suitable | technique(s) FISH: suitable | technique(s) - | technique(s) - |
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename - |
| packaging kit of 1 (5 components) | packaging kit of 1 (7 components) | packaging pkg of 25 μL (250 nmol; 10mM) | packaging - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Kit Components Only
Product No.
Description
- Blocking Reagent (green screw cap)
- Phosphate Buffered Saline (PBS) (blue screw cap)
- D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS)
- Dimethylsulfoxide (DMSO) (red screw cap)
- Sephadex G-25 Columns
Storage Class
11 - Combustible Solids
wgk
WGK 1
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 11418165001 | 04061838701794 |