11418165001

Roche

Biotin Protein Labeling Kit

Quality Level

usage

sufficient for 5 labeling reactions

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

General description

For labeling of proteins with biotin.
Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.

Application

Biotin protein labeling kit has been used to produce biotinylated denatured bovine serum albumin (d-BSA) for coating cadmium telluride (CdTe) quantum dot (QD) conjugates.
The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.

Packaging

1 kit containing 5 components.

Quality

Function tested.

Preparation Note

Working solution: Preparation of the solutions
  • Blocking solution: Dissolve the contents of the bottle blocking reagent (green screw cap) in 300 ml double-distilled water.
  • PBS solution: Dissolve the contents of the bottle PBS (blue screw cap) in 1 I double-distilled water.
  • Biotin-7-NHS solution: The solution should be freshly prepared before labeling. Add to the contents of the reaction vial which includes biotin-7-NHS 250 μI DMSO and vortex the solution several times. The resulting concentration of the biotin-7-NHS solution is 20 mg/ml. For the labeling mix, the volume of the biotin-7-NHS solution should not exceed 5% of the total reaction volume (e.g., add maximal 50 μl biotin-7-NHS solution to 1 ml protein solution).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • Blocking Reagent (green screw cap)

  • Phosphate Buffered Saline (PBS) (blue screw cap)

  • D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS)

  • Dimethylsulfoxide (DMSO) (red screw cap)

  • Sephadex G-25 Columns

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Certificate of Analysis

Certificate of Origin

Oladapo O Yeku et al.
Frontiers in immunology, 12, 663379-663379 (2021-05-04)
Immunotherapy for ovarian cancer is an area of intense investigation since the majority of women with relapsed disease develop resistance to conventional cytotoxic therapy. The paucity of safe and validated target antigens has limited the development of clinically relevant antibody-based...
Simultaneous and sensitive determination of multiplex chemical residues based on multicolor quantum dot probes.
Peng C, et al.
Biosensors And Bioelectronics, 24(12), 3657-3662 (2009)
Xing Wei et al.
Stem cells (Dayton, Ohio), 27(2), 478-488 (2008-11-22)
Adipose tissue stroma contains a population of mesenchymal stem cells, which support repair when administered to damaged tissues, in large part through secreted trophic factors. We directly tested the ability of media collected from cultured adipose-derived stem cells (ASCs) to...
Xingwang Zhao et al.
Science advances, 5(7), eaaw0315-eaaw0315 (2019-07-23)
B cell activation is regulated by the stimulatory or inhibitory co-receptors of B cell receptors (BCRs). Here, we investigated the signaling mechanism of Fc receptor-like 1 (FcRL1), a newly identified BCR co-receptor. FcRL1 was passively recruited into B cell immunological...

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