11418467001

Chymotrypsin Sequencing Grade

from bovine pancreas

• Synonym(s): chymotrypsin, protease
• EC Number: 3.4.21.1 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: bovine pancreas
• Quality Level: 100
• assay: ≥90%
• form: lyophilized (salt-free)
• specific activity: ≥70 units/mg protein (at 25 °C, with ATEE as a substrate)
• mol wt: M_r 25 kDa
• packaging: pkg of 4 × 25 μg
• manufacturer/tradename: Roche
• technique(s): protein sequencing: suitable
• optimum pH: 7.0-9.0
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Chymotrypsin Sequencing Grade is isolated as a specific protease in pure form from bovine pancreas.

Specificity

Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-terminial of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate.[1] Acts also upon amides and esters of susceptible amino acids. The specificity of Chymotrypsin Sequencing Grade is tested with melittin as a substrate.

Application

Use Chymotrypsin Sequencing Grade for the hydrolysis of proteins by chymotrypsin alone or in combination with other proteases.[2] It is suitable for peptide mapping, fingerprinting, and sequence analysis.

Quality

Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.

Preparation Note

Working concentration: The recommended amount of enzyme is 1/200 to 1/20 of the quantity of protein by weight.
Storage conditions (working solution): 2 to 8 °C
A solution in 1 mM HCl can be stored for up to one week at 2 to 8 °C.
Inhibitors: Aprotinin, DFP, PMSF, phenothiazine-N-carbonyl chloride, TPCK, ZPCK, α2-macroglobulin, α1-antitrypsin, soybean trypsin inhibitor, and chymostatin. No inhibition by APMSF.

Reconstitution

Dissolve lyophilized chymotrypsin sequencing grade in 1mM HCl. The proteins to be sequenced are dissolved in digestion buffer (100mM Tris-HCl, 10mM CaCl₂, pH 7.8).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• pictograms: GHS07,GHS08
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable