11420470001

dUTP

=98%, solution, 100 mM, suitable for DNA amplification

• Synonym(s): 2′-deoxy-uridine-5′-triphosphate, deoxy-uridine-5′-triphosphate, 2′-

Properties

• description: Lithium salt
• Assay: 98%
• form: solution
• usage: sufficient for 6,250 standard reactions (PCR)
• packaging: pkg of 250 μL (25 μmol; 100 mM)
• manufacturer/tradename: Roche
• concentration: 100 mM
• technique(s): DNA amplification: suitable; DNA sequencing: suitable
• storage temp.: −20°C

Description

General description

dUTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).

Contents
dUTP solution in water (pH 7), 100 mM, lithium salt

Application

DNA that contains dUTP is susceptible to hydrolysis with Uracil-DNA Glycosylase. Therefore, dUTP is most commonly used (e.g., in PCRs and RT-PCRs with Taq DNA Polymerase) to prevent carryover contamination.However, dUTP is also a substrate for other DNA synthesizing enzymes (e.g., the Kornberg DNA polymerase and the Klenow Enzyme). Therefore, dUTP can be used for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

Quality

Typical analysis:

• ≥98% dUTP (HPLC), =1.5% dUDP (HPLC), =0.5% dUMP (HPLC)
• Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀

Unit Definition

1 μmol dUTP = 9.9 A₂₆₀ units

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): does not flash
• Flash Point(C): does not flash