11427598910
Roche
Biotin-16-ddUTP
≥98% (HPLC), solution, 1 mM, suitable for hybridization
Synonym(s):
Bio-16-ddUTP tetralithium salt, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′,3′-dideoxyuridine 5′-triphosphate
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25 μL
$513.00
$513.00
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About This Item
Empirical Formula (Hill Notation):
C32H52N7O17P3S
CAS Number:
Molecular Weight:
931.78
MDL number:
UNSPSC Code:
41116100
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Quality Level
assay
≥98% (HPLC)
form
solution
mol wt
955.5 (biotin-16-ddUTP-Li4)
packaging
pkg of 25 μL (25 nmol; 1mM)
manufacturer/tradename
Roche
concentration
1 mM
technique(s)
hybridization: suitable
storage temp.
−20°C
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Related Categories
1 of 4
This Item | 11093070910 | 11051482001 | 11051458001 |
|---|---|---|---|
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form solution | form solution | form solution | form solution |
| assay ≥98% (HPLC) | assay - | assay ≥98% | assay 98% |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| concentration 1 mM | concentration - | concentration 100 mM | concentration 100 mM |
General description
Biotin-16-ddUTP, tetralithium salt; 1mM
Application
- Biotin-16-ddUTP is used as a substrate for: Terminal Transferase
- DNA polymerase I (Holoenzyme and Klenow fragment)
- T4 DNA polymerase
- Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor).
- The biotin-labeled oligonucleotide can be used as a hybridization probe for: DNA and RNA transfers
- Colony and plaque screening
- In situ hybridization
The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.
Oligonucleotides are enzymatically labeled at their 3′ end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine- triphosphate (biotin-ddUTP).
It has been used in the biotinylation of in vitro transcribed tmRNA during surface plasmon resonance (SPR) technique.[3] It has been used in the standard nick translation reaction for tumor DNA labelling during comparative genomic hybridization.[4]
Features and Benefits
Biotin is bound to dideoxyuridine triphosphate via an amide linkage.
Analysis Note
Typical analysis: At least 85% Biotin-16-ddUTP (HPLC, area%).
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
does not flash
flash_point_c
does not flash
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