11427598910
Roche
Biotin-16-ddUTP
≥98% (HPLC), solution, 1 mM, suitable for hybridization
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Synonym(s):
Bio-16-ddUTP tetralithium salt, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′,3′-dideoxyuridine 5′-triphosphate
Empirical Formula (Hill Notation):
C32H52N7O17P3S
Recommended Products
Quality Level
assay
≥98% (HPLC)
form
solution
mol wt
955.5 (biotin-16-ddUTP-Li4)
packaging
pkg of 25 μL (25 nmol; 1mM)
manufacturer/tradename
Roche
concentration
1 mM
technique(s)
hybridization: suitable
storage temp.
−20°C
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General description
Biotin-16-ddUTP, tetralithium salt; 1mM
Application
- Biotin-16-ddUTP is used as a substrate for: Terminal Transferase
- DNA polymerase I (Holoenzyme and Klenow fragment)
- T4 DNA polymerase
- Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor).
- The biotin-labeled oligonucleotide can be used as a hybridization probe for: DNA and RNA transfers
- Colony and plaque screening
- In situ hybridization
The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.
Oligonucleotides are enzymatically labeled at their 3′ end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine- triphosphate (biotin-ddUTP).
It has been used in the biotinylation of in vitro transcribed tmRNA during surface plasmon resonance (SPR) technique. It has been used in the standard nick translation reaction for tumor DNA labelling during comparative genomic hybridization.
Features and Benefits
Biotin is bound to dideoxyuridine triphosphate via an amide linkage.
Quality
Typical analysis: At least 85% Biotin-16-ddUTP (HPLC, area%).
Other Notes
For life science research only. Not for use in diagnostic procedures.
wgk_germany
nwg
flash_point_f
does not flash
flash_point_c
does not flash
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van de Vliet M S, et al.
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