Roche

Peroxidase (POD), activated

Name: Peroxidase (POD), activated
Brand: ROCHE
Price: 677 USD

from horseradish

Synonym(s):

POD

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8 MG

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About This Item

EC Number:

1.11.1.7 (BRENDA, IUBMB)

UNSPSC Code:

12352204

Key Documents

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biological source

horseradish

Quality Level

100

form

lyophilized

specific activity

800 units/mg protein (at 25 °C with ABTS and H₂O₂ as substrate (pH 5.0).)

packaging

pkg of 8 mg (~40 mg lyophilizate)

manufacturer/tradename

Roche

optimum pH

6.0-6.5

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Proteins & Enzymes

Roche Life Science Products

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This Item	10108090001	P1709	77332
Roche 11428861001 Peroxidase (POD), activated	Roche 10108090001 Peroxidase (POD)	Sigma-Aldrich P1709 Peroxidase, Maleimide Activated from horseradish	Sigma-Aldrich 77332 Peroxidase from horseradish
biological source horseradish	biological source horseradish	biological source horseradish	biological source horseradish
specific activity 800 units/mg protein (at 25 °C with ABTS and H₂O₂ as substrate (pH 5.0).)	specific activity ≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.), 250 U/mg	specific activity >200 units/mg protein	specific activity ~150 U/mg
form lyophilized	form lyophilized (salt-free)	form lyophilized powder	form powder
shipped in wet ice	shipped in wet ice	shipped in -	shipped in wet ice
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
optimum pH 6.0-6.5	optimum pH 6.0-6.5	optimum pH -	optimum pH -

General description

Donor: hydrogen-peroxide oxidoreductase
Reagent for the labeling of water-soluble substances carrying primary amino groups with peroxidase from horseradish (HRP).
Capacity: sufficient for approximately 6 mg IgG.

Peroxidase (POD) is an enzyme that includes a group of specific enzymes such as NAD peroxidase, NADP peroxidase, fattyacid peroxidase and a group of non specific enzymes from various sources. It is present in animals, higher plants and other organisms.[1]

Application

Peroxidase (POD), activated has been used in terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labeling (TUNEL) staining[2][3] and immunohistochemistry.[4]

The enzyme can be used without pre-activation for labeling water-soluble substances with reactive and accessible primary amino groups (e.g., peptides or proteins) with peroxidase for use in analytical methods. It is particularly suitable for the coupling of antibodies (Ig, Ig Fab, and Ig F(ab′)₂ fragments from rabbit, mouse, sheep, and goat) with peroxidase. The resulting conjugate is optimal for use in immunochemical detection systems, for example, ELISA, immunohistochemistry, and immunoblotting procedures.

Biochem/physiol Actions

Peroxidase (POD) helps in the dehydrogenation of several organic compounds like phenols, aromatic amines, hydroquinones etc.[1]

Preparation Note

Dissolving the lyophilizate in 0.5 ml double-dist. Water produces a peroxidase concentration of 16 mg/ml.

Analysis Note

Isoenzymes: >90% isoenzyme C (HPLC)
Purity number: 3.0 - 3.5 (A₄₀₃/A₂₇₅)

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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