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11449451910

Roche

DNA Molecular Weight Marker VIII, DIG-labeled

greener alternative

pkg of 500 μL (10 μg/ml), solution, 10 μg/mL

Synonym(s):

DNA marker

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About This Item

UNSPSC Code:
41105335

form

solution

Quality Level

packaging

pkg of 500 μL (10 μg/ml)

manufacturer/tradename

Roche

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

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concentration

10 μg/mL

greener alternative category

storage temp.

−20°C

General description

Fragment mixture prepared by cleavage of pUCBM21 DNA with Hpa II, and pUCBM21 DNA cleaved with Dra I and Hind III.
Size Range: 19 to 1114 bp
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Sequence

The mixture contains 17 DNA fragments with the following base pair lengths: 19, 26, 34/34, 37, 67, 110, 124, 147, 190, 242, 320, 404, 489, 501, 692, 900, and 1114 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Physical form

Ready-to-use solution in 10mM Tris-HCl, 1mM EDTA, pH 8.0.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

does not flash

flash_point_c

does not flash


Certificates of Analysis (COA)

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Bing Luo et al.
World journal of gastroenterology, 11(5), 629-633 (2005-01-19)
To understand the expression of latent and lytic genes of Epstein-Barr virus (EBV) in EBV-associated gastric carcinoma (EBVaGC) and to explore the relationship between EBV-encoded genes and development of EBVaGC at molecular level. One hundred and seventy-two gastric carcinoma tissues

Articles

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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