11449460001

DNA Molecular Weight Marker IX

solution, pkg of 50 μg (in 200 μl)

• Synonym(s): DNA marker
• UNSPSC Code: 41105335
• NACRES: NA.54
• Form: solution
• Solubility: water: miscible
• Storage temp.: −20°C

Properties

• form: solution
• packaging: pkg of 50 μg (in 200 μl)
• manufacturer/tradename: Roche
• concentration: 250 μg/mL
• solubility: water: miscible
• color: colorless
• storage temp.: −20°C
• Quality Level: 100

Description

General description

DNA Molecular Weight Marker IX contains a mixture of eleven DNA fragments. The mixture is obtained by cleavage of φX 174 DNA with restriction endonuclease Hae III.
Size Range: 72 to 1353 bp

Application

DNA Molecular Weight Marker IX is especially designed for the analysis of mid-size PCR products in agarose gels.[1] It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:[2]

• Restriction digests
• PCR
• RT-PCR

Preparation Note

Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.

Other Notes

50 μg = 1A₂₆₀ unit

For life science research only. Not for use in diagnostic procedures.

The mixture contains eleven DNA fragments with the following base pair lengths (1 base pair = 660 Daltons): 72, 118, 194, 234, 271, 281, 310, 603, 872, 1078, and 1353.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: does not flash
• flash_point_c: does not flash