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Key Documents

11579681001

Roche

RNase, DNase-free, High Concentration

from bovine pancreas

Synonym(s):

Rnase

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About This Item

UNSPSC Code:
41105600

biological source

bovine pancreas

Quality Level

form

solution

specific activity

≥30 U/mg

packaging

pkg of 1 mg (10 mg/ml)

manufacturer/tradename

Roche

concentration

10 mg/mL

General description

A heterogeneous mixture of ribonucleases specially prepared to be free of deoxyribonuclease activity according to the current Quality Control procedures.

Application

High concentration, DNase-free RNase has been used for:
  • Genomic DNA isolation
  • Isolation of plasmid DNA
  • Degradation of RNA for flow cytometry

Quality

Absence of DNase activity tested according to the current Quality Control procedures.

Unit Definition

One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions: A0 to A1 corresponds to the total conversion, A1 being the final absorbance.

Preparation Note

Working concentration: 50 μg/ml is the recommended working concentration of RNase, DNase-free, for the isolation of genomic DNA.
Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0

Other Notes

For life science research only. Not for use in diagnostic procedures.
Unlike most RNase preparations, no boiling before use is necessary to remove DNase activity.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


Certificates of Analysis (COA)

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