MilliporeSigma
All Photos(3)

Documents

11585606910

Roche

DIG-High Prime

greener alternative

sufficient for 40 labeling reactions, pkg of 160 μL, solution

Sign Into View Organizational & Contract Pricing

Synonym(s):
DIG system

form

solution

Quality Level

usage

sufficient for 40 labeling reactions

packaging

pkg of 160 μL

manufacturer/tradename

Roche

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative category

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
112770659100335357591011363514910
DIG-High Prime sufficient for 40 labeling reactions, pkg of 160 μL, solution

Roche

11585606910

DIG-High Prime

DIG DNA Labeling Mix

Roche

11277065910

DIG DNA Labeling Mix

packaging

pkg of 160 μL

packaging

pkg of 50 μL

packaging

-

packaging

kit of 1 (5 components)

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

form

solution

form

solution

form

-

form

-

General description

DIG-High Prime has enzyme and nucleotide mixture for rapid random-primed labeling of DNA with Digoxigenin-11-dUTP. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. DIG-labeled probes are generated at high yield within one hour or after overnight incubation.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Specificity

Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or by heating to 65 °C for 10 minutes.

Application

DIG-High Prime-labeled DNA probes has been used in a variety of hybridization techniques :
  • in Southern blots
  • in northern blots
  • in dot/slot blots
  • for screening of gene libraries
  • in In situ hybridizations
Due to highly specific and sensitive detection systems, DIG-labeled DNA probes can be used for single-copy gene detection in 1μg total human DNA. The use of the alkali-labile form of DIG-dUTP, in which the digoxigenin moiety is connected to the spacer arm via an alkali-labile ester bond, enables easier and more efficient stripping and reprobing of blots.

Features and Benefits

DIG-High Prime guarantees efficient labeling of:
  • DNA amounts ranging from 10ng to 3μg in a standard reaction.
  • DNA of different lengths ranging from small restriction fragments to λ or cosmid DNA.
  • DNA, supercoiled or linearized.
  • DNA in low melting-point agarose.

Labeling efficiency:
A standard labeling reaction with 1μg template yields 0.8μg newly synthesized digoxigenin-labeled DNA after 1 hour, and 2μg after a 20-hour incubation at +37°C.

Contents
5x concentrated random primer mix: 1U/ μl Klenow polymerase, labeling grade, 1mM dATP, 1mM dCTP, 1mM dGTP, 0.65mM dTTP, 0.35mM DIG-11-dUTP, alkali labile in 50% (v/v) glycerol.

Quality

Function test:
In a standard assay with 1μg linearized pBR 328, 0.8μg of DIG-labeled DNA is synthesized after 1 hour, and 2.3μg after 20 hours. When this labeled DNA is used for hybridization at a concentration of 20ng/ml, 0.03pg homologous DNA are detected by chemiluminescence with the anti-DIG-alkaline phosphatase conjugate and CSPD on a dot or Southern blot.

Principle

DIG-labeled DNA probes are generated with DIG-High Prime according to the random-primed labeling technique. DIG-High Prime is a specifically developed reaction mixture containing Digoxigenin-11-dUTP and all reagents necessary for random-primed labeling, including Klenow enzyme, premixed in an optimized 5x concentrated reaction buffer in 50% glycerol.

Preparation Note

DIG-labeled probes in the reaction mix or in the hybridization buffer are stable for more than 12 months stored at -15 to -25°C. They can be reused several times if freshly denatured before use.

Assay Time: 80 minutes

Sample Materials
  • DNA fragments of at least 100bp
  • Linearized plasmid, cosmid or λDNA
  • Supercoiled DNA
  • Or minimal amounts of DNA (10ng), e.g., DNA restriction fragments isolated from gels or in molten agarose

Note: To obtain optimal results, template DNA should be linearized and should have a size of = 100bp or larger. Template DNA > 5kb should be restriction-digested using a 4bp cutter prior to labeling.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 5

1 of 5

Light regulation of asexual development in the rice blast fungus, Magnaporthe oryzae
<BIG>Lee K, et al.</BIG>
Fungal genetics and biology : FG & B, 43 (2006)
Characterization of the Chromosomal Transmission of Intergeneric Hybrids of spp. and by Genomic in situ Hybridization.
Wang X
Crop Science, 50, 1642-1648 (2010)

Articles

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service