11644793001

Cytotoxicity Detection Kit (LDH)

suitable for protein quantification, suitable for cell analysis, detection, sufficient for ≤2,000 tests

• Synonym(s): LDH

Properties

• usage: sufficient for ≤2,000 tests
• Quality Level: 100
• packaging: pkg of 1 kit
• manufacturer/tradename: Roche
• technique(s): protein quantification: suitable
• λmax: 490 nm
• application(s): cell analysis; detection
• detection method: colorimetric
• storage temp.: −20°C

Description

General description

A nonradioactive alternative to the [⁵¹Cr] and [³H]-thymidine release assay.
Colorimetric assay for the quantification of cell death and cell lysis, based on the measurement of lactate dehydrogenase (LDH) activity released from the cytosol of damaged cells into the supernatant.

Application

The Cytotoxicity Detection Kit (LDH) is a fast and simple method to quantify cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well plate format. Thus, the kit can be used in many different in vitro cell systems when damage to the plasma membrane occurs. For example:

• Detection and quantification of cell-mediated cytotoxicity
• Determination of mediator-induced cytolysis
• Determination of the cytotoxic potential of compounds in environmental and medical research, and in the food, cosmetic, and pharmaceutical industries
• Determination of cell death in bioreactors
• Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth

Packaging

1 kit containing 2 components.

Preparation Note

Working solution: Vial 1 Catalyst:
Reconstitute the lyophilisate in 1 ml double dist. water for 10 minutes and mix thoroughly.

Vial 2 Dye solution:
Ready-to-use solution.

Reaction mixture
For 100 tests: Shortly before use, mix 250 μl of vial 1 with 11.25 ml of vial 2.
For 400 tests: Shortly before use, add the total volume of catalyst (1 ml) to the total volume
of dye solution (45 ml) and mix well.

Note: The Reaction mixture should not be stored; prepare immediately before use.
Storage conditions (working solution): 2 to 8 °C
The lyophilizate is stable at 2 to 8 °C.
The reconstituted catalyst solution is stable for four weeks when stored at 2 to 8 °C.
Once thawed, the dye solution is stable for several weeks when stored at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

• Catalyst (Diaphorase/NAD⁺ mixture)
• Dye Solution (INT and sodium lactate)

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash