11696505001

Roche

Agarose Gel DNA Extraction Kit

Quality Level

manufacturer/tradename

Roche

packaging

kit of for up to 100 reactions

General description

For the elution of DNA fragments from agarose gels.

The Agarose Gel DNA Extraction Kit provides convenient isolation of high-quality DNA in large amounts.
By combining agarose gel electrophoresis and extraction, you can easily concentrate dilute, aqueous DNA solutions. After processing with the kit, the DNA can be recovered in a volume of 20 to 50 μL. Isolated DNA fragments are free of inhibitors that could affect common downstream procedures. For example, recovered DNA can be efficiently ligated into cloning vectors or labeled to high specific activity. Restriction digests proceed without inhibition.

Application

Agarose Gel DNA Extraction Kit has been used to extract full-length (CHRNA_v2) and shortest (CHRNA_v3) amplicons from agarose gels.
The Agarose DNA Extraction Kit efficiently isolates both small and large DNA fragments from standard or low melting point agarose. Recovered DNA fragments are suitable for:

  • Ligation and transformation
  • Enzymatic restriction
  • Random primed or nick translation labeling methods
  • Sequencing
  • PCR/long PCR
  • Cloning
  • Concentrate dilute nucleic acid solutions

Features and Benefits

  • Quick and simple.
Extract high yields of DNA in only 45 minutes, with few hands-on steps.
  • Combine with different agaroses and buffer systems.
Low melting point agarose is not required.
  • Purify efficiently.
Highly specific binding of DNA allows easy removal of impurities.
  • Isolate large DNA fragments without shearing.
Silica particles are uniform in size and have smooth surfaces, ensuring recovery of intact DNA fragments up to 100 kb.
  • Isolate oligonucleotides >=20 bp.
Narrow size distribution of the silica particles and absence of fines ensure high binding capacity of the matrix.
  • Avoid enzymatic inhibition.
No fine particles are observed in the suspension. The recovered product will not inhibit subsequent enzymatic reactions.

Components

  • Silica Matrix, for 100 standard reactions
  • Solubilization Buffer
  • Nucleic Acid Binding Buffer
  • Wash Buffer

Quality

DNA Molecular Weight Marker II is separated in a 0.8% agarose gel and the 546-, 2,322-, and 6,557 bp fragments are isolated with the kit components. The expected amount of DNA is recovered for each fragment. Depending on parameters such as fragment length, preparation, and purity of the applied DNA, up to 80% recovery is achieved. DNA fragments are digested with restriction enzymes. No inhibition of DNA digestion is observed.

Preparation Note

A solubilization buffer containing a chaotropic salt (sodium perchlorate) dissolves an agarose gel slice that contains a DNA fragment. In the presence of the chaotropic salt, the DNA fragment binds selectively to silica matrix. The DNA remains bound while a series of rapid wash-and-spin steps remove contaminating small molecules. Finally, low-salt elution removes the DNA from the silica matrix. The process does not require DNA precipitation, organic solvent extractions, or extensive handling of the DNA.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Flame over circleExclamation mark

Signal Word

Danger

Hazard Statements

RIDADR

UN 1502 5.1 / PGII

WGK Germany

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Zhongchun Tong et al.
Experimental and therapeutic medicine, 14(6), 5491-5496 (2017-12-30)
A high prevalence of Enterococcus faecalis (E. faecalis) is observed in teeth with root canal treatment failures. Clustered regularly interspaced short palindromic repeats (CRISPR) are widely distributed in prokaryotes that have adaptive immune systems against mobile elements, including pathogenic genes....
Joris van Arensbergen et al.
Nature biotechnology, 35(2), 145-153 (2016-12-27)
Previous methods to systematically characterize sequence-intrinsic activity of promoters have been limited by relatively low throughput and the length of the sequences that could be tested. Here we present 'survey of regulatory elements' (SuRE), a method that assays more than...
Sahika Cingir Koker et al.
PloS one, 13(12), e0208982-e0208982 (2018-12-14)
Cholinergic Receptor Nicotinic Alpha 5 (CHRNA5) is an important susceptibility locus for nicotine addiction and lung cancer. Depletion of CHRNA5 has been associated with reduced cell viability, increased apoptosis and alterations in cellular motility in different cancers yet not in...
Cholinergic Receptor Nicotinic Alpha 5 (CHRNA5) RNAi is associated with cell cycle inhibition, apoptosis, DNA damage response and drug sensitivity in breast cancer
Koker SC, et al.
PLoS ONE, 13(12), e0208982-e0208982 (2018)
Li-Li Guo et al.
Experimental and therapeutic medicine, 15(6), 5394-5402 (2018-05-31)
Viral vectors represent a potential strategy for the treatment of human malignant tumors. Currently, recombinant adenovirus vectors are commonly used as gene therapy vehicles, as it possesses a proven safety profile in normal human cells. The recombinant adenovirus system has...

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