Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

11699075001

Roche

Nylon Membranes for Colony and Plaque Hybridization

Synonym(s):

membrane

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41105339

packaging

pkg of 50 discs (82 mm diameter)

Quality Level

manufacturer/tradename

Roche

disc diam.

82 mm

storage temp.

20-25°C

General description

Nylon membrane is a circular, uncharged, microporous, amphoteric, ultra-pure nylon 66 membrane on a polyester support. It has a durable structure with consistent transfer rate.
Surface Properties: Pore size of 1.2 μm. Surface contains 50% amino and 50% carboxyl groups.

Application

  • The membrane discs are specifically suited for:Nonradioactive screening of phage or cosmid libraries with digoxigenin-labeled probes followed by detection with highly sensitive chemiluminescent or chromogenic substrates
  • Hybridization with biotinylated or radioactive probes
  • It has been used in the colony immunoblotting technique.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

K P Karey et al.
Analytical biochemistry, 178(2), 255-259 (1989-05-01)
Western blotting of low molecular weight (Mr) acidic and basic isoelectric point (pI) proteins was studied to optimize detection sensitivities. Radioiodinated epidermal growth factor (EGF, Mr 6045, pI 4.4), transforming growth factor type alpha (TGF alpha, Mr 5623, pI 6.8)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service