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11719386001

Roche

Immunoprecipitation Kit (Protein G)

sufficient for 20 reactions, kit of 1, suitable for immunoprecipitation (IP)

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Synonym(s):
Protein G, immunoprecipitation kit

usage

sufficient for 20 reactions

packaging

kit of 1

manufacturer/tradename

Roche

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Kit contains all reagents necessary for cell lysis, solubilization, stabilization, and immunopurification of proteins.

Specificity

Protein G is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein G binds nearly all subclasses of IgG, but no other classes of immunoglobulins.

Application

The immunoprecipitation kit has been used for the immunoprecipitation of proteins from cellular extracts with Protein G Agarose.

Packaging

1 kit containing 5 components.

Preparation Note

Working solution: Lysis buffer/wash buffer 1

The kit contains reagents for 125 ml of lysis buffer/wash buffer 1. Prepare at least a minimal volume of 25 ml, sufficient for four immunoprecipitations.
To prepare 25 ml of lysis buffer/wash buffer 1 mix 5 ml core buffer, 3.75 ml NaCl, 2.5 ml detergent mix and 1 cOmplete Tablet. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C for 24 hours. When stored in aliquots at -15 to -25 °C, the solution is stable for at least four weeks. Mix thoroughly after thawing.

Wash buffer 2

The kit contains reagents for 50 ml of wash buffer 2. 2 ml of this buffer is required for one immunoprecipitation.
To prepare 50 ml of wash buffer 2 mix 10 ml core buffer, 25 ml NaCl and 0.5 ml detergent mix. Add water to a final volume of 50 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.

Wash buffer 3

The kit contains reagents for 25 ml of wash buffer 3. 1 ml of this buffer is required for one immunoprecipitation.
To prepare 25 ml mix 1 ml core buffer and 0.25 ml detergent mix. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • Core Buffer

  • NaCl

  • Detergent Mix

  • cOmplete Protease Inhibitor Cocktail Tablets (5)

  • Protein G Agarose ready-to-use

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class

8B - Non-combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_c

does not flash


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