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11734334001

Roche

Universal Protease Substrate

lyophilized, suitable for detection, pkg of 40 mg (40 μmol; 100mM)

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Synonym(s):
Substrate of protease, casein, resorufin-labeled, protease substrate, universal
UNSPSC Code:
12352204

form

lyophilized

Quality Level

packaging

pkg of 40 mg (40 μmol; 100mM)

manufacturer/tradename

Roche

concentration

1 mg/mL

application(s)

detection

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

The Universal Protease Substrate provides rapid and highly sensitive detection of trace protease activity, or determination of the effectiveness of protease inhibition. Protease activity releases resorufin-labeled peptides from the patented substrate, resorufin-labeled casein. Resorufin is N-(resorufin-4-carbonyl)-piperidine-4-carbonic acid).The concentration of these labeled peptides in the supernatant is directly related to the proteolytic activity present.
  • Conveniently detect nanogram quantities of proteolytic activity in less than one hour.
  • Perform highly sensitive protease detection in a homogeneous assay.

Application

Principle
By treatment with proteases, resorufin-labeled peptides are released from resorufin-labeled casein. They cannot be precipitated by trichloroacetic acid. The concentration of these resorufin-labeled peptides in the supernatant is equivalent to the proteolytic activity present.
Use Universal Protease Substrate as a general substrate for proteases, and to detect trace protease activities, for example, as a contamination in enzyme preparations. It can be measured spectrophotometrically and fluorimetrically in a homogeneous assay.

Quality

Function test: performance-controlled in the assay for proteases.

Preparation Note

Working concentration: Approximately 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
An aqueous solution is stable for several months at -15 to -25° C and for 2 to 3 days at 2 to 8° C. It is recommended to store aqueous solutions in aliquots at -15 to -25° C. Repeated freezing and thawing is possible. At 15 to 25 °C the product is rapidly hydrolyzed in solution.

Other Notes

For life science research only. Not for use in diagnostic procedures.

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Differential Proinflammatory Responses to Aspergillus fumigatus by Airway Epithelial Cells In Vitro Are Protease Dependent
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Airborne Fungi Induce Nasal Polyp Epithelial Cell Activation and Toll-Like Receptor Expression
Shin, et al.
International Archives of Allergy and Immunology, 153 (1), 46-52 (2010)
Anna V Luebben et al.
Science advances, 8(15), eabj8633-eabj8633 (2022-04-16)
Genetic CLN5 variants are associated with childhood neurodegeneration and Alzheimer's disease; however, the molecular function of ceroid lipofuscinosis neuronal protein 5 (Cln5) is unknown. We solved the Cln5 crystal structure and identified a region homologous to the catalytic domain of

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