11745816910
Roche
DIG-Nick Translation Mix
sufficient for 40 labeling reactions, pkg of 160 μL, suitable for hybridization
Synonym(s):
nick translation
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$799.00
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About This Item
$799.00
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form
solution
Quality Level
usage
sufficient for 40 labeling reactions
packaging
pkg of 160 μL
manufacturer/tradename
Roche
greener alternative product characteristics
Designing Safer Chemicals
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sustainability
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technique(s)
hybridization: suitable
greener alternative category
, Aligned
storage temp.
−20°C
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This Item | 11745808910 | 11745824910 | 11277073910 |
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usage sufficient for 40 labeling reactions | usage sufficient for 50 labeling reactions | usage sufficient for 40 labeling reactions | usage sufficient for 20 reactions |
technique(s) hybridization: suitable | technique(s) nucleic acid labeling: suitable | technique(s) hybridization: suitable | technique(s) - |
form solution | form solution | form solution | form solution |
packaging pkg of 160 μL | packaging pkg of 200 μL | packaging pkg of 160 μL | packaging pkg of 40 μL |
manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche |
greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics - | greener alternative product characteristics - | greener alternative product characteristics - |
General description
Specificity
Application
Note: The mix can also be used for filter hybridization techniques, however, for highly sensitive filter hybridization probes, we recommend that you use DIG-High Prime from Roche Applied Science.
For nonradioactive labeling of in situ probes with other haptens and fluorophores, Roche Applied Science offers the Biotin-Nick Translation Mix and the Nick Translation Mix (without nucleotides).
Quality
Principle
E. coli DNA Polymerase I synthesizes DNA complementary to the intact strand in a 5′→3′ direction using the 3′-OH termini of the nick as a primer. The 5′→3′ exonucleolytic activity of DNA polymerase I simultaneously removes nucleotides in the direction of synthesis. The polymerase activity sequentially replaces the removed nucleotides with isotope-labeled or hapten-labeled deoxyribonucleoside triphosphates. At low temperature (+15°C), the unlabeled DNA in the reaction is thus replaced by newly synthesized labeled DNA.
Preparation Note
Assay Time: 100 minutes
Sample Materials: Supercoiled and linearized plasmid DNA, Supercoiled and linearized cosmid DNA, Purified PCR products.
Note: Denaturing of the template before nick translation is not required.
Other Notes
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
No data available
flash_point_c
No data available
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