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11767305001

Roche

TUNEL Enzyme

from calf thymus recombinant in E. coli

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Synonym(s):
transferase dUTP nick end labeling, tunel

recombinant

expressed in E. coli

Quality Level

100

form

solution

usage

sufficient for 20 tests

packaging

pkg of 2 × 50 μL

manufacturer/tradename

Roche

storage temp.

−20°C

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This Item
11767291910S7107QIA33
TUNEL Enzyme from calf thymus recombinant in E. coli

Roche

11767305001

TUNEL Enzyme

TUNEL Label Mix sufficient for 30 tests, pkg of 3 × 550 μL

Roche

11767291910

TUNEL Label Mix

ApopTag TdT Enzyme ApopTag Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3′-OH ends of fragmented DNA during apoptotic cell detection.

Sigma-Aldrich

S7107

ApopTag TdT Enzyme

Sigma-Aldrich

Sigma-Aldrich

QIA33

FragEL DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme

form

solution

form

solution

form

-

form

-

packaging

pkg of 2 × 50 μL

packaging

pkg of 3 × 550 μL

packaging

-

packaging

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

usage

sufficient for 20 tests

usage

sufficient for 30 tests

usage

-

usage

sufficient for 50 tests

General description

TUNEL enzyme solution, containing terminal deoxynucleotidyl transferase from calf thymus recombinant in E. coli.
One of the two components required for performing the TUNEL reaction to detect apoptosis in situ.

Application

The enzyme solution contains terminal deoxynucleotidyl transferase from calf thymus, recombinant in E. coli, needed to perform the TUNEL reaction for detecting apoptosis in situ.
The TUNEL reaction mixture is used to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.

Physical form

Terminal deoxynucleotidyl transferase (TdT) in an optimized concentration, dissolved in reaction buffer. Ready-to-use with TUNEL Label.

Preparation Note

Working solution: TUNEL Enzyme is used in combination with TUNEL Label to prepare the TUNEL reaction mixture.
To produce a TUNEL solution sufficient for one test:
  • Mix 5μl TUNEL Enzyme with 45μl TUNEL Label prior to use.
  • For negative control use 50μl/test TUNEL Label only.

Storage conditions (working solution): Note: The TUNEL reaction mixture (45μl TUNEL Label with 5μl TUNEL Enzyme for 1 test) should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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