11767305001

Roche

TUNEL Enzyme

from calf thymus recombinant in E. coli

Quality Level

form

solution

usage

sufficient for 20 tests

packaging

pkg of 2 × 50 μL

manufacturer/tradename

Roche

shipped in

dry ice

storage temp.

−20°C

General description

TUNEL enzyme solution, containing terminal deoxynucleotidyl transferase from calf thymus recombinant in E. coli.
One of the two components required for performing the TUNEL reaction to detect apoptosis in situ.

Application

The enzyme solution contains terminal deoxynucleotidyl transferase from calf thymus, recombinant in E. coli, needed to perform the TUNEL reaction for detecting apoptosis in situ.
The TUNEL reaction mixture is used to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.

Physical form

Terminal deoxynucleotidyl transferase (TdT) in an optimized concentration, dissolved in reaction buffer. Ready-to-use with TUNEL Label.

Preparation Note

Working solution: TUNEL Enzyme is used in combination with TUNEL Label to prepare the TUNEL reaction mixture.
To produce a TUNEL solution sufficient for one test:
  • Mix 5μl TUNEL Enzyme with 45μl TUNEL Label prior to use.
  • For negative control use 50μl/test TUNEL Label only.

Storage conditions (working solution): Note: The TUNEL reaction mixture (45μl TUNEL Label with 5μl TUNEL Enzyme for 1 test) should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.

Other Notes

For life science research only. Not for use in diagnostic procedures.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Yajuan Guo et al.
eLife, 9 (2020-05-06)
The intermediate filament protein keratin 14 (K14) provides vital structural support in basal keratinocytes of epidermis. Recent studies evidenced a role for K14-dependent disulfide bonding in the organization and dynamics of keratin IFs in skin keratinocytes. Here we report that...
Mathilde Bouchet et al.
Cancer research, 80(13), 2914-2926 (2020-05-06)
Bone is the most common metastatic site for breast cancer. Although the estrogen-related receptor alpha (ERRα) has been implicated in breast cancer cell dissemination to the bone from the primary tumor, its role after tumor cell anchorage in the bone...

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