11796895001

DIG Easy Hyb^™ Granules

powder, suitable for hybridization

• Synonym(s): DIG system
• UNSPSC Code: 41105326
• NACRES: NA.54

Properties

• agency: suitable for
• form: powder
• usage: sufficient for 6 x 100 mL
• manufacturer/tradename: Roche
• storage condition: (Keep container tightly closed in a dry and well-ventilated place.)
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): hybridization: suitable
• color: white
• pH: 7,0
• solubility: water: soluble
• suitability: suitable for nucleic acid hybridization
• greener alternative category: Aligned,
• storage temp.: 20-25°C

Description

General description

DIG Easy Hyb^™ is a hybridization solution used for nucleic acid blots with digoxigenin-labeled probes. DIG Easy Hyb does not contain formamide but facilitates the lowering of hybridization temperature comparable to buffers that contain 50% formamide.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG Easy Hyb Granules can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes.[1][2][3] It is specifically designed for use with DIG kits.

Features and Benefits

• DIG Easy Hyb^™ is a convenient ready-to-use hybridization buffer, saving time and effort.
• DIG Easy Hyb aligns with the principles of Green Chemistry by offering a greener alternative for hybridization buffers.

Preparation Note

Working solution: Add carefully 64 ml sterile double-dist. water to the plastic bottle, dissolve by stirring immediately for 5 minutes at 37 °C. The availability of 6 x 100 ml portions allows you to freshly "produce" the DIG Easy Hyb when required—no long-term storage of a solution is required.
Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.

Nontoxic granules, aliquoted in plastic bottles. Reconstitute to provide 100ml of a 1x solution, ready-to-use.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

DIG Easy Hyb is a trademark of Roche

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319
• pcodes: P264 - P280 - P302 + P352 - P332 + P313 - P337 + P313 - P362 + P364
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: does not flash
• flash_point_c: does not flash