11814320001

Roche

Formamide

Synonym(s):
Amide C1, Formic amide, Formamide
Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
Beilstein/REAXYS Number:
505995
MDL number:
PubChem Substance ID:

Quality Level

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

assay

≥99%

form

liquid

autoignition temp.

932 °F

mol wt

45.04

packaging

pkg of 500 mL

manufacturer/tradename

Roche

application(s)

hybridization: suitable

color

colorless

refractive index

n20/D 1.447 (lit.)

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

density

1.134 g/mL at 25 °C (lit.)

absorption

≤0.08 at 290 nm

shipped in

wet ice

storage temp.

2-8°C

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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General description

Formamide is an organic solvent, which allows for the denaturation and renaturation of nucleic acids at room temperature. This is particularly useful for protocols where reaction times are long and high temperatures would damage biological activity through chain scissions and depurination etc. Formamide reduces thermal stability of double stranded nucleic acids and is generally used for DNA renaturation or DNA-RNA hybridization. Specificity and rate of reaction are determined by formamide concentration and temperature of the reaction.

Application

Formamide has been used in:
in situ hybridization
  • as a component of 2X denaturing loading dye for RNA polyacrylamide gel electrophoresis
  • for the extraction of evans blue dye in hindlimb of mice in vascular leakage assay
  • as a component of stop buffer used in cleavage and polyadenylation activity assays

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Quality

Contaminants: ≤5 ppm heavy metals (as Pb), ≤5 ppm Fe

Other Notes

For life science research only. Not for use in diagnostic procedures.

hazcat

Carc. 2 - STOT RE 2

storage_class_code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 1

Flash Point(F)

305.6 °F

Flash Point(C)

152 °C

Certificate of Analysis

Certificate of Origin

mRNA deadenylation is coupled to translation rates by the differential activities of Ccr4-Not nucleases
Webster MW, et al.
Molecular Cell, 70(6), 1089-1100 (2018)
Impaired collateral vessel formation in sickle cell disease
Okwan-Duodu D, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 38(5), 1125-1133 (2018)
Protocols for two-and three-color fluorescent RNA in situ hybridization of the main and accessory olfactory epithelia in mouse
Ishii T, et al.
Journal of Neurocytology, 33(6), 657-669 (2004)
Nucleic acid reassociation in formamide.
B L McConaughy et al.
Biochemistry, 8(8), 3289-3295 (1969-08-01)
Activation of the Endonuclease that Defines mRNA 3? Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex
Hill CH, et al.
Molecular Cell, 73(6), 1217-1231 (2019)

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