11814770001

Roche

DNA Isolation Kit for Cells and Tissues

Quality Level

manufacturer/tradename

Roche

packaging

kit of for 10 isolations

General description

The DNA Isolation Kit for Cells and Tissues provides medium- and large-scale preparation of purified genomic DNA ranging in size from 50 to 150 kb. Remove contaminating RNA and proteins from a wide variety of biological specimens (mammalian tissue, cultured cells, yeast, gram-negative bacteria or mouse tail).

Application

DNA Isolation Kit for Cells and Tissues has been used to isolate DNA from a wide variety of starting materials. The isolated DNA is suitable for many molecular biology applications:
  • Genomic Southern blotting
  • Sequencing
  • Restriction digestion
  • PCR/long PCR
  • Cloning

Features and Benefits

Obtain increased yields of DNA in less than 2.5 hours.
Benefit from a simple, straightforward procedure (compared with column based methods).
Increase safety and convenience.
Eliminate the use of chaotropic salts, anion-exchange columns, and hazardous organic solvents.
Reduce purification time.
All required reagents are included in the kit.

Components

  • Cellular Lysis Buffer
  • Proteinase K Solution
  • RNase Solution
  • Protein Precipitation Solution

Quality

Each lot of the DNA Isolation Kit for Cells and Tissues is tested for the absence of DNase contamination. Function tests to purify DNA from bovine liver, followed by specific amplification with the Expand High Fidelity PCR System were performed.

Preparation Note

Sample material is homogenized in Cellular Lysis Buffer in the presence of a strong anionic detergent and proteinase K. RNA is eliminated by RNase treatment and proteins are removed by selective precipitation and centrifugation. The purified DNA is finally recovered from solution by isopropanol precipitation.

Other Notes

Figure 1: Amplification of both short and long DNA fragments from genomic DNA prepared with the DNA Isolation Kit for Cells and Tissues. Genomic DNA was isolated from a variety of sources and then amplified with either Taq DNA Polymerase, the Expand High Fidelity PCR System, or the Expand Long Template PCR System.
Lanes 2, 3: Human DMD fragment (268 bp) and mouse c-myc fragment (580 bp), amplified with Taq DNA Polymerase
Lanes 4, 5, 7, 8: Human c-myc fragment (1.2 kb), mouse β2-microglobulin fragment (3.6 kb), bovine lysozyme gene fragment (6.9 kb), and human tPA gene fragment (9.3 kb), all amplified with the Expand High Fidelity PCR System
Lanes 6, 9, 10: Mouse α-2 collagen gene fragments (5.6 kb and 10.4 kb) and human β-globin fragment (23 kb), amplified with the Expand Long Template PCR System
Lanes 1, 11 : DNA Molecular Weight Markers VI and II
For life science research only. Not for use in diagnostic procedures.
Purity of isolated DNA: Average A 260 / A 280 ratio: 1.7 - 1.9

Isolation of High Molecular Weight DNA
The kit simplifies the isolation of 50 to 150 kb genomic DNA.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

hazcat

Eye Irrit. 2

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Soolmaz Mousavi et al.
The journal of venomous animals and toxins including tropical diseases, 20, 51-51 (2014-01-01)
Despite the high importance of Helicobacter pylori, the origin and transmission of this bacterium has not been clearly determined. According to controversial theories and results of previous studies, animal source foods - especially milk - play an important role in...
Reza Ranjbar et al.
FEBS open bio, 6(5), 433-441 (2016-07-16)
Despite the clinical importance of Helicobacter pylori in human gastric disorders, its exact route of transmission is still uncertain. Based on the contentious hypothesis and findings of previous investigations, water may play an important role in the transmission of H....
Ehsan Razmara et al.
BMC cardiovascular disorders, 18(1), 137-137 (2018-07-05)
Myosin VI, encoded by MYH6, is expressed dominantly in human cardiac atria and plays consequential roles in cardiac muscle contraction and comprising the cardiac muscle thick filament. It has been reported that the mutations in the MYH6 gene associated with...
VacA, cagA, iceA and oipA genotypes status and antimicrobial resistance properties of Helicobacter pylori isolated from various types of ready to eat foods
Hemmatinezhad B, et al.
Annals of Clinical Microbiology and Antimicrobials, 15(1), 2-2 (2016)
Ehsan Razmara et al.
Iranian journal of basic medical sciences, 21(3), 333-341 (2018-03-08)
Targeted next-generation sequencing (NGS) provides a consequential opportunity to elucidate genetic factors in known diseases, particularly in profoundly heterogeneous disorders such as non-syndromic hearing loss (NSHL). Hearing impairments could be classified into syndromic and non-syndromic types. This study intended to...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service