11814770001

Roche

DNA Isolation Kit for Cells and Tissues

Quality Level

manufacturer/tradename

Roche

packaging

kit of for 10 isolations

General description

The DNA Isolation Kit for Cells and Tissues provides medium- and large-scale preparation of purified genomic DNA ranging in size from 50 to 150 kb. Remove contaminating RNA and proteins from a wide variety of biological specimens (mammalian tissue, cultured cells, yeast, gram-negative bacteria or mouse tail).

Application

DNA Isolation Kit for Cells and Tissues has been used to isolate DNA from a wide variety of starting materials. The isolated DNA is suitable for many molecular biology applications:
  • Genomic Southern blotting
  • Sequencing
  • Restriction digestion
  • PCR/long PCR
  • Cloning

Features and Benefits

Obtain increased yields of DNA in less than 2.5 hours.
Benefit from a simple, straightforward procedure (compared with column based methods).
Increase safety and convenience.
Eliminate the use of chaotropic salts, anion-exchange columns, and hazardous organic solvents.
Reduce purification time.
All required reagents are included in the kit.

Components

  • Cellular Lysis Buffer
  • Proteinase K Solution
  • RNase Solution
  • Protein Precipitation Solution

Quality

Each lot of the DNA Isolation Kit for Cells and Tissues is tested for the absence of DNase contamination. Function tests to purify DNA from bovine liver, followed by specific amplification with the Expand High Fidelity PCR System were performed.

Preparation Note

Sample material is homogenized in Cellular Lysis Buffer in the presence of a strong anionic detergent and proteinase K. RNA is eliminated by RNase treatment and proteins are removed by selective precipitation and centrifugation. The purified DNA is finally recovered from solution by isopropanol precipitation.

Other Notes

Figure 1: Amplification of both short and long DNA fragments from genomic DNA prepared with the DNA Isolation Kit for Cells and Tissues. Genomic DNA was isolated from a variety of sources and then amplified with either Taq DNA Polymerase, the Expand High Fidelity PCR System, or the Expand Long Template PCR System.
Lanes 2, 3: Human DMD fragment (268 bp) and mouse c-myc fragment (580 bp), amplified with Taq DNA Polymerase
Lanes 4, 5, 7, 8: Human c-myc fragment (1.2 kb), mouse β2-microglobulin fragment (3.6 kb), bovine lysozyme gene fragment (6.9 kb), and human tPA gene fragment (9.3 kb), all amplified with the Expand High Fidelity PCR System
Lanes 6, 9, 10: Mouse α-2 collagen gene fragments (5.6 kb and 10.4 kb) and human β-globin fragment (23 kb), amplified with the Expand Long Template PCR System
Lanes 1, 11 : DNA Molecular Weight Markers VI and II
For life science research only. Not for use in diagnostic procedures.
Purity of isolated DNA: Average A 260 / A 280 ratio: 1.7 - 1.9

Isolation of High Molecular Weight DNA
The kit simplifies the isolation of 50 to 150 kb genomic DNA.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Fatemeh Bitarafan et al.
Journal of clinical laboratory analysis, 34(12), e23544-e23544 (2020-08-31)
The extremely high genetic heterogeneity of hearing loss due to diverse group of genes encoding proteins required for development, function, and maintenance of the complex auditory system makes the genetic diagnosis of this disease challenging. Up to now, 121 different...
Lindsay H Levkoff et al.
Neoplasia (New York, N.Y.), 10(8), 804-816 (2008-08-07)
The thymidine analog bromodeoxyuridine (BrdU) is incorporated into newly synthesized DNA and has been shown to increase the susceptibility of incorporating cells to ionizing radiation. However, in the absence of secondary stressors, BrdU is thought to substitute relatively benignly for...
Shahrzad Atapoor et al.
Jundishapur journal of microbiology, 7(5), e10013-e10013 (2014-08-26)
There is a possibility for the presence of Helicobacter pylori in vegetables due to their close contact with polluted water, soil and feces. This study was carried out to detect the presence of H. pylori in vegetables and salads in...
Mehdi Bamorovat et al.
Iranian journal of parasitology, 9(3), 342-349 (2015-02-14)
Canine visceral leishmaniasis (CVL) is a systemic disease with a high mortality rate, caused by a diphasic protozoan parasite, Leishmania infantum/chagasi in the world. The objective of the present study was to determine the presence of CVL in the city...
Elnaz Saeidi et al.
BioMed research international, 2016, 8701067-8701067 (2016-04-19)
According to controversial theories and results of studies, foods with animal origins play an important role in the transmission of H. pylori to human. The aim of this study was to determine the distribution of vacA genotypes of H. pylori...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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