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11836170001

Roche

cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail

Protease Inhibitor Cocktail Tablets provided in a glass vial, Tablets provided in a glass vial

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Synonym(s):
protease inhibitor, protease inhibitor cocktail, roche protease inhibitor

form

tablet

usage

sufficient for 25 10mL extraction solution preparations

packaging

vial of 25 tablets

manufacturer/tradename

Roche

technique(s)

inhibition assay: suitable

solubility

aqueous buffer: soluble

storage temp.

2-8°C

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This Item
COEDTAF-ROCO-ROS8830
form

tablet

form

tablet

form

tablet

form

tablet

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

packaging

vial of 25 tablets

packaging

vial of 20 tablets (11873580001), vial of 3 × 20 tablets (05056489001)

packaging

vial of 20 tablets (11697498001), vial of 3 × 20 tablets (11836145001)

packaging

-

solubility

aqueous buffer: soluble

solubility

aqueous buffer: soluble

solubility

aqueous buffer: soluble

solubility

-

usage

sufficient for 25 10mL extraction solution preparations

usage

-

usage

-

usage

-

General description

cOmplete, Mini, EDTA-free Protease Inhibitor Tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other cOmplete tablets, they do not contain EDTA, leaving the stability and the function of metal-dependant proteins unaffected. The affinity purification of Poly-His-tagged fusion proteins using IMAC (Immobilized Metal Affinity Chromatography) is also facilitated (no dialysis necessary).
Due to the optimized composition of the tablets, they show excellent inhibition of serine and cysteine proteases, and well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete, Mini, EDTA-free tablets, contains both irreversible and reversible protease inhibitors. Metallo- and aspartic proteases are not inhibited.

cOmplete Mini, EDTA-free Tablets are identical to cOmplete Mini Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.

Specificity

For the inhibition of serine, cysteine, but not metalloproteases.

Application

cOmplete, Mini, EDTA-free protease inhibitor cocktail has been used for the inhibition of serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts.
cOmplete, Mini, EDTA-free Tablets, are used for the inhibition of proteolytic activity in small volumes (up to 10 ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, Mini, EDTA-free, is preferentially used in the isolation process of Poly-His-tagged fusion proteins.
If it is necessary to inhibit proteolytic activity in a large volume (up to 50 ml), we recommend to use cOmplete, EDTA-free.
cOmpleteMini, EDTA-free Protease Inhibitor Cocktail has used as a component of
  • resuspension buffer for nuclear lysates preparation from prostate cancer cell lines for chromatin and RNA immunoprecipitation
  • resuspension buffer for protein extraction from neurospheres for western blot analysis
  • homogenization buffer for protein extraction from rat left ventricle samples and immunoprecipitaion buffer for human embryonic kidney 293 (HEK293) cells

Features and Benefits

Use cOmplete, Mini Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results.

  • Easy-to-use: Simply drop a quick-dissolving tablet into your buffer.
  • Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
  • Complete protection: Instantly protect your proteins against a broad range of proteases.
  • Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.

Components

Proprietary mixture of several protease inhibitors.

Quality

Performance-tested with pancreas extract.

Physical form

Tablets in glass vials

Preparation Note

Working concentration: 1 tablet per 10 ml extraction solution
Working solution: Preparation of Working Solution:

One cOmplete Mini EDTA-free tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively a stock solution (7 X conc.) can be prepared.

Stock solution (7 X conc.):

Dissolve one cOmplete Mini EDTA-free tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.

Reconstitution

Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C, or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C.

Legal Information

cOmplete is a trademark of Roche

also commonly purchased with this product

Product No.
Description
Pricing

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash


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Patrick R Punch et al.
Glycobiology, 30(7), 446-453 (2020-01-04)
High-dose irradiation poses extreme risk of mortality from acute damage to the hematopoietic compartment and gastrointestinal tract. While bone marrow transplantation can reestablish the hematopoietic compartment, a more imminent risk of death is posed by gastrointestinal acute radiation syndrome (GI-ARS)
Protein phosphatase 1c associated with the cardiac sodium calcium exchanger 1 regulates its activity by dephosphorylating serine 68-phosphorylated phospholemman
Hafver T, et al.
The Journal of biological chemistry, 291(9), 4561-4579 (2016)
Donald B Jump
Methods in molecular biology (Clifton, N.J.), 579, 375-389 (2009-09-19)
Very long chain fatty acids confer functional diversity on cells by variations in their chain length and degree of unsaturation. Microsomal fatty acid elongation represents the major pathway for determining the chain length of saturated, monounsaturated, and polyunsaturated fatty acids
Risk SNP-mediated promoter-enhancer switching drives prostate cancer through lncRNA PCAT19
Hua JT, et al.
Cell, 174(3), 564-575 (2018)
Melissanne de Wispelaere et al.
Journal of virology, 86(13), 7072-7083 (2012-04-26)
The dengue virus (DV) envelope (E) protein is important in mediating viral entry and assembly of progeny virus during cellular infection. Domains I and III (DI and DIII, respectively) of the DV E protein are connected by a highly conserved

Articles

Get maximum protection after protein isolation by using protease and phosphatase inhibitor cocktail tablets from Roche.

Protocols

Dissolve one tablet in 10 ml aqueous buffer or water. If high proteolytic activity is present, dissolve one tablet in 7 ml buffer.

Related Content

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

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