MilliporeSigma
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11855476001

Roche

Titan One Tube RT-PCR System

sufficient for 100 reactions, kit of 1 (4 components), suitable for RT-PCR, hotstart: no

Synonym(s):
RT-PCR

Quality Level

usage

sufficient for 100 reactions

feature

dNTPs included: no
hotstart: no

packaging

kit of 1 (4 components)

manufacturer/tradename

Roche

parameter

42-60 °C optimum reaction temp.

technique(s)

RT-PCR: suitable

input

purified RNA

shipped in

dry ice

storage temp.

−20°C

General description

The Titan One Tube RT-PCR System uses Reverse Transcriptase (RT) Avian Myeloblastosis Virus (AMV) for first strand cDNA synthesis and the Expand High Fidelity enzyme blend consisting of Taq DNA Polymerase and a polymerase with a proofreading activity for amplification of cDNA by polymerase chain reaction (PCR). The proofreading activity, leads to an increased PCR fidelity. The Titan One Tube RT-PCR System:
  • Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparision to Taq DNA polymerase.
  • Delivers increased sensitivity due to the high efficiency of all three enzymes.
  • Is faster than conventional two-step RT-PCR.

Specificity

Specificity of the reaction strongly depends on primer design and DNA contamination.
To exclude artefacts from DNA targets appropriate positive and negative control reaction should be included.
Heat inactivation: 2 min at 94 °C
Reverse Transcriptase AMV

Application

Titan One Tube RT-PCR System has been used:
  • to amplify the regions containing the miRNA target (miRT) inserts/response elements in viral RNA
  • for determining the presence or absence of RNA templates
  • in cloning of rare messages without the need to construct cDNA libraries
  • for quantifying the levels of gene expression

Features and Benefits

Titan One Tube RT-PCR System:
  • High Fidelity: Expand High Fidelity enzyme blend with proofreading activity reduces the error rate in polymerase chain reaction (PCR)
  • offers a threefold higher fidelity in comparison to Taq DNA polymerase
  • delivers increased sensitivity due to the high efficiency of all three enzymes
  • faster than conventional two-step reverse transcriptase (RT)-PCR
  • allows the amplification of full-length cDNA up to 6 kb by RT-PCR due to the use of the Expand PCR System

Packaging

1 kit containing 4 components

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under such patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
Expand is a trademark of Roche
Titan is a trademark of Roche

Kit Components Only

Product No.
Description

  • Reverse Transcriptase AMV and Expand High Fidelity Enzyme Mix in storage buffer

  • RT-PCR Reaction Buffer 5x concentrated

  • MgCl2 Stock Solution, 25 mM

  • DTT Solution, 100 mM

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

Noura B Elsedawy et al.
Molecular therapy oncolytics, 17, 484-495 (2020-06-13)
Infectious nucleic acid has been proposed as a superior formulation for oncolytic virus therapy. Oncolytic picornaviruses can be formulated as infectious RNA (iRNA), and their unwanted tropisms eliminated by microRNA (miRNA) detargeting. However, genomic insertion of miRNA target sequences into
Autumn J Ruiz et al.
Journal of virology, 90(8), 4078-4092 (2016-02-13)
Mengovirus, a member of thePicornaviridaefamily, has a broad cell tropism and can cause encephalitis and myocarditis in multiple mammalian species. Attenuation has been achieved by shortening the polycytidine tract in the 5' noncoding region (NCR). A poly(C)-truncated strain of mengovirus
Yogesh R Suryawanshi et al.
Molecular therapy oncolytics, 23, 1-13 (2021-10-01)
A dual microRNA-detargeted oncolytic Mengovirus, vMC24NC, proved highly effective against a murine plasmacytoma in an immunocompetent syngeneic mouse model; however, there remains the concern of escape mutant development and the potential for toxicity in severely immunocompromised cancer patients when it

Protocols

Titan™ One Tube RT-PCR System Protocol & Troubleshooting

Titan One Tube RT-PCR System Protocol & Troubleshooting

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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