MilliporeSigma
All Photos(3)

Documents

12140306001

Roche

GC-RICH PCR System

sufficient for ≤50 reactions, pkg of 100 U, optimum reaction temp. 72 °C

Sign Into View Organizational & Contract Pricing

Synonym(s):
GC-RICH PCR System, GC-rich | PCR

usage

sufficient for ≤50 reactions

Quality Level

packaging

pkg of 100 U

manufacturer/tradename

Roche

parameter

72 °C optimum reaction temp.

technique(s)

PCR: suitable

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
EHIFIP-ROEHIFI-ROEHIFIDNTP-RO
vibrant-m

12140306001

GC-RICH PCR System

usage

sufficient for ≤50 reactions

usage

sufficient for ≤2,500 reactions (03300234001), sufficient for 2500 reactions, sufficient for ≤500 reactions (03300226001), sufficient for 500 reactions

usage

sufficient for ≤1,000 reactions (11759078001), sufficient for ≤200 reactions (11732650001), sufficient for ≤40 reactions (11732641001)

usage

sufficient for ≤1,000 reactions (04738276001), sufficient for ≤200 reactions (04738268001), sufficient for ≤40 reactions (04738250001)

packaging

pkg of 100 U

packaging

pkg of 2,500 U (03300234001 [10x250 U]), pkg of 500 U (03300226001 [2x250 U])

packaging

pkg of 2,500 U (11759078001 [10x250 U]), pkg of 100 U (11732641001), pkg of 500 U (11732650001 [2x250 U])

packaging

pkg of 2,500 U (04738276001 [10x250 U]), pkg of 100 U (04738250001), pkg of 500 U (04738268001 [2x250 U])

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

parameter

72 °C optimum reaction temp.

parameter

72 °C optimum reaction temp.

parameter

-

parameter

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

The GC-RICH PCR System is composed of an enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3′-5′ exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields, fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR Reaction buffer in combination with the separately included GC-RICH resolution solution allows to amplify difficult templates like GC-rich targets very efficiently. TA cloning is recommended. The enzyme blend produces more blunt-ended fragments than Taq DNA Polymerase. The majority of products have single A overhangs. Use 2 U for a standard 50 μl PCR.

Contents

  • GC-RICH Enzyme Mix , in storage buffer
  • GC-RICH Reaction Buffer, 5x concentrated, with 7.5 mM MgCl2 and DMSO
  • GC-RICH Resolution Solution, 5 M
  • MgCl2 Stock Solution, 25 mM
  • Water, PCR Grade

Application

GC-RICH PCR system has been used in semi‐quantitative analysis, genotyping and retrieval and cloning of high-GC content sequence of candidate gene.
The GC-RICH PCR System, a blend of Taq DNA polymerase and a proofreading polymerase, enables amplification of templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superb performance – especially from problematic templates.
The GC-RICH PCR System may also be used in standard PCR applications, providing improved results (higher yield, higher accuracy) over Taq DNA polymerase alone.

Advantages of the GC-Rich PCR System:

  • Ease access to difficult templates, including GC-rich targets and repetitive sequences.
  • Reagents, the GC-RICH Resolution Solution and PCR Grade Water are provided.
  • Amplify DNA fragments up to 5 kb.

Features and Benefits

Blend of Taq DNA Polymerase and a proofreading polymerase amplifies difficult templates. Enhanced processivity and the unique GC-RICH Resolution Solution deliver superior performance.

  • PCR
  • Difficult templates up to 5 kb

Use the GC-Rich PCR System, dNTPack with ready-to-use PCR Nucleotide Mix.
The GC-RICH PCR System is a blend of Taq DNA Polymerase and a proofreading polymerase for amplifying longer nucleic acid fragments. The GC-Rich Solution provides an solution to enable amplification of all kind of difficult PCR products.
When using the supplied magnesium-containing buffer, the final MgCl2 concentration is 1.5 mM.

Packaging

1 kit containing 5 components

Quality

The GC-RICH PCR System is function-tested, by amplifying a human 284 bp ApoE genomic fragment, using the GC-RICH PCR System protocols

Unit Definition

Volume Activity: 2 U/μl

Preparation Note

Working concentration: Enzyme concentration
The optimal enzyme concentration range from 0.5 to 5 U per assay. For a standard 50 μl PCR, we recommend using 2 U of the enzyme blend.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

Kit Components Only

Product No.
Description

  • GC-RICH Enzyme Mix, in storage buffer

  • GC-RICH PCR Reaction Buffer, with 7.5 mM MgCl2 and DMSO 5x concentrated

  • GC-RICH Resolution Solution 5 M

  • MgCl2 Stock Solution 25 mM

  • Water, PCR Grade

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 7

1 of 7

G J Chang et al.
Genetics and molecular research : GMR, 14(3), 8509-8515 (2015-09-09)
In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification
Micro RNA?132/212 family enhances arteriogenesis after hindlimb ischaemia through modulation of the Ras?MAPK pathway.
Lei Z, et al.
Journal of Cellular and Molecular Medicine, 19(8), 1994-2005 (2015)
Induction of autophagy with catalytic mTOR inhibitors reduces huntingtin aggregates in a neuronal cell model.
Roscic A, et al.
Journal of Neurochemistry, 119(2), 398-407 (2011)
Zhiyong Lei et al.
Frontiers in physiology, 11, 590-590 (2020-07-03)
Background: Myocardial infarction (MI) is caused by occlusion of the coronary artery and induces ischemia in the myocardium and eventually a massive loss in cardiomyocytes. Studies have shown many factors or treatments that can affect the healing and remodeling of
C Ronald Scott et al.
The Journal of pediatrics, 163(2), 498-503 (2013-03-08)
To assess the performance of a tandem mass spectrometry (MS/MS) technology in a newborn screening laboratory to simultaneously measure α-galactosidase, acid-α-glucosidase, and α-L-iduronidase for the detection of infants at risk to develop Fabry, Pompe, or mucopolysaccharidosis (MPS)-I diseases. Enzyme activity

Articles

GC-RICH Amplification of Polymerase Chain Reaction (PCR) System Troubleshooting.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service