MilliporeSigma
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APTAT-RO

Roche

AptaTaq Fast DNA Polymerase

pkg of 100 U (06879110001), pkg of 1,000 U (06879128001)

Synonym(s):
AptaTaq

packaging

pkg of 1,000 U (06879128001)
pkg of 100 U (06879110001)

Quality Level

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

General description

AptaTaq Fast DNA Polymerase is a thermostable, recombinant Taq polymerase with aptamer mediated, reversible hot start. It is immediate activated by heating, ideal for specific priming and fast PCR. Comes with optimized buffer system. Allows reaction setup at room temperature. A one-for-all protocol delivers superb results in less than 30 min for a broad range of templates incl. GC-rich templates.
AptaTaq Fast DNA Polymerase is optimized for fast end-point polymerase chain reaction assays. Enzyme and reaction buffer composition are optimized for fast activation and short PCR reaction times. AptaTaq Fast DNA Polymerase is optimized for high specificity by minimizing the formation of non-specific amplification products.
Similar to antibody-based methods, the enzyme is activated by heating. Dropping the temperature below +55°C acts like a switch, shutting off polymerase activity. At temperatures above +60°C, AptaTaq Polymerase is activated, ideal for specific priming and fast PCR.
It is a Kit for fast end-point PCR.
Save time
  • 4 minutes processing time only.
  • Immediate enzyme activation.
Amplify with Confidence
  • One-for-all protocol.
  • Room temperature setup.
  • Prevent carry-over contamination.
AptaTaq Fast Polymerase & Master redefine fast PCR:
  • AptaTaq Fast products are ready-to-use reagents optimized for fast PCR on all thermal cyclers. Inbuilt aptamer mediated hot start assures high specificity.

Application

AptaTaq Fast DNA Polymerase is a thermostable, recombinant Taq polymerase with aptamer mediated, reversible hot start & immediate activation. Similar to antibody-based methods, the enzyme is activated by heating, ideal for specific priming and fast PCR.

  • Fast Hot Start PCR optimized for PCR products up to 500 bp
  • Fast Hot Start RT-PCR optimized for PCR products up to 500 bp
For maximum convenience, select the ready-to-use AptaTaq Fast PCR Master.

Packaging

1 kit containing 4 components

Unit Definition

One unit AptaTaq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of deoxyribonucleosidetriphosphates into acid precipitable DNA within 60 min at +65 °C.

Volume Activity: 2.5 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.
Save time
  • 4 minutes processing time only.
  • Immediate enzyme activation.

Amplify with Confidence
  • One-for-all protocol.
  • Room temperature setup.
  • Prevent carry-over contamination.

AptaTaq Fast Polymerase & Master redefine fast PCR:
  • AptaTaq Fast products are ready-to-use reagents optimized for fast PCR on all thermal cyclers. Inbuilt aptamer mediated hot start assures high specificity.

Legal Information

NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.</ br>80: NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent Nos. 5,670,637, 5,693,502, and corresponding patent claims outside the US.  No right under any other patent claim is conveyed expressly, by implication, or by estoppel.
AptaTaq is a trademark of Roche

Kit Components Only

Product No.
Description

  • AptaTaq Fast DNA Polymerase

  • AptaTaq Fast DNA Polymerase Buffer 5x concentrated

  • MgCl2 25 mM

  • Water, PCR Grade

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Quotes and Ordering

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