COUEDTAF-RO
Roche
cOmplete™ ULTRA Tablets, EDTA-free, glass vials Protease Inhibitor Cocktail
Tablets provided in glass vials
Recommended Products
form
tablet
usage
sufficient for 50 mL (05892953001)
sufficient for 50 mL (06538282001)
packaging
pkg of 2 × 10 tablets (05892953001)
pkg of 6 × 10 tablets (06538282001)
manufacturer/tradename
Roche
solubility
aqueous buffer: soluble
absorption
0.4 at 280 nm (1x solution)
storage temp.
2-8°C
Related Categories
General description
cOmplete ULTRA Tablets EDTA-free are identical to cOmplete ULTRA Tablets, the only difference being that no EDTA or other chelating agents (e.g. EGTA) are included.
Contents
Tablets in a glass vial. Each tablet is sufficient for a volume of 50ml extraction solution.
Specificity
Application
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine and cysteine proteases. cOmplete ULTRA Tablets were developed for improved efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
This tablet does not contain EDTA; therefore metalloproteases are not inhibited.
- ice-cold radioimmunoprecipitation assay buffer (RIPA) for the lysis of retinal ganglion cells
- lysis buffer for green fluorescent protein (GFP)- cell-penetrating peptides (CPPs) fusion protein isolation from E coli
- homogenization buffer for mice protein isolation from intestinal tissue
Features and Benefits
- Protect your precious proteins.
- Why settle for less.
- Defend against a broad range of proteases.
- Convenient and safe tablet format.
into your buffers.
Components
Physical form
Preparation Note
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not your fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One tablet is sufficient for the inhibition of proteolytic activity in 50 ml extraction solution. If very high proteolytic activity is present, 2-3 tablets should be used for 50 ml extraction buffer. The tablets can be added directly to the extraction medium.
Stock Solution (2x conc.)
Alternatively to the direct use with lysates, a 2x stock solution can be prepared, with the following procedure:
• Add one cOmplete ULTRA Tablet, EDTA-free to 25 ml double-distilled water in a PE or PP tube.
• Vortex 4 min, until the tablet is dissolved.
• The stock solution is ready to use even if there are very fine particles still visible in solution. These particles do not interfere with the inhibition performance of the tablets.
• This stock solution is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
• During storage of the 2x stock solution, the particles can sediment. Briefly vortex the stock solution prior to use.
Storage conditions (working solution): The stock solution in water is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
Reconstitution
Other Notes
Legal Information
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Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
Storage Class
8A - Combustible, corrosive hazardous materials
wgk_germany
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Get maximum protection after protein isolation by using protease and phosphatase inhibitor cocktail tablets from Roche.
Related Content
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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