T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.
Heat inactivation: The enzyme can be inactivated by heating (65 °C, 10 minutes).
Ligation of sticky- and blunt-ended DNA fragments.
Tested for the absence of deoxyribonucleases and exonucleases, according to the current Quality Control procedures.
One unit of T4 DNA ligase is the amount of enzyme activity that converts 1 nmol 32P from pyrophosphate into Norit-absorbable material in 20 minutes at +37 °C. One unit corresponds to 0.2 U determined in the exonuclease III resistance assay. Dilution buffer: 50 mM Tris-HCl, 10 mM dithioerythritol, bovine serum albumin, 500 μg/ml, pH 7.6 (+25 °C).
Volume Activity: 1 x 103 U/ml; 5 x 103 U/ml
Activator: Mg2+ (10mM), DTT
Working solution: For dilution of the enzyme Roche recommends using a buffer containing the components of the storage buffer: 20 mM Tris-HCl, 60 mM KCl, 1 mM EDTA, 5 mM dithoerythritol, 50% glycerol (v/v), pH 7.5 (4 °C).
For life science research only. Not for use in diagnostic procedures.