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EHIFIPN-RO

Roche

Expand High FidelityPLUS PCR System, dNTPack

sufficient for ≤125 reactions (04743725001), sufficient for ≤500 reactions (04743733001), suitable for PCR, dNTPs included

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Enzyme Commission number:

grade

Molecular Biology

Quality Level

form

liquid

usage

sufficient for ≤125 reactions (04743725001)
sufficient for ≤500 reactions (04743733001)

feature

High Fidelity PCR
dNTPs included
hotstart: no

packaging

pkg of 125 U (04743725001)
pkg of 500 U (04743733001 [2x250 U])

manufacturer/tradename

Roche

concentration

50 U/mL (working concentration)

parameter

72 °C optimum reaction temp.

technique(s)

PCR: suitable

impurities

ribonucleases, none detected
≤50 cfu/mL bioburden

color

colorless

input

purified DNA

solubility

water: miscible

storage temp.

−20°C

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This Item
EHIFIP-ROEHIFIDNTP-ROEHIFI-RO
Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

form

liquid

form

-

form

-

form

-

concentration

50 U/mL (working concentration)

concentration

-

concentration

-

concentration

-

solubility

water: miscible

solubility

-

solubility

-

solubility

-

General description

The Expand High FidelityPLUS PCR System is a blend of Taq DNA Polymerase and a thermostable proofreading protein that lacks polymerase activity.
This enzyme blend is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity.
In addition, this enzyme blend also incorporates dUTP and, in combination with Uracil-DNA Glycosylase, can be used to safeguard PCR reactions from cross contamination.
The fidelity of DNA synthesis with the Expand High FidelityPLUS PCR System shows a 2-fold increase compared to the fidelity obtained with the Expand High Fidelity PCR System and a 6-fold increase compared to the fidelity obtained with Taq DNA Polymerase.
These characteristics make the Expand High FidelityPLUS PCR System blend the product of choice if a larger number of targets need to be amplified at the same time with high yield in combination with improved accuracy. In addition, the product can be used for the efficient labeling of DNA fragments with radioactive or non-radioactive modified nucleotides.

Application

  • The Expand High FidelityPLUS PCR System is designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This convenient dNTPack is the product of choice for the development of a new amplification assay or the optimization of an existing assay. PCR
  • RT-PCR
  • Primer extension
  • Carryover prevention
It has been used in the ultradeep pyrosequencing methods, inverse PCR and to measure the repeat length of CGG sequences.
Twofold higher yield and threefold greater fidelity are obtained compared to Taq DNA Polymerase. Use Expand High FidelityPLUS PCR System for:
  • PCR up to 5kb
  • RT-PCR up to 5kb

Features and Benefits

Expand High FidelityPLUS PCR System is a mixture of Taq DNA polymerase and a DNA polymerase with proofreading activity. The Expand High FidelityPLUS PCR System shows a twofold increase in fidelity compared to the Expand High Fidelity PCR System, and a sixfold increase compared to Taq DNA Polymerase.
By incorporating dUTP, Uracil-DNA Glycosylase can be used to prevent PCR product carryover. Use this kit for efficient labeling of DNA with radioactive or non-radioactive nucleotides. It is optimized for PCR of DNA fragments up to 5kb.
Use 2.5U for a standard 50μl PCR.

  • Safeguard against cross-contamination.
This is the choice for large numbers of amplifications.
  • Obtain sixfold higher fidelity and higher yields,
compared to Taq DNA Polymerase.
  • Efficiently label DNA.
Use this kit to label DNA with radioactive or nonradioactive nucleotides.
  • Cost-effective.
Use the convenient solution of premixed PCR grade dNTPs.

Packaging

1 kit containing 5 components

Quality

Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.

Unit Definition

Volume Activity: 5 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Expand is a trademark of Roche

Kit Components Only

Product No.
Description

  • Expand High FidelityPLUS Enzyme Blend 5 U/μl

  • Expand High FidelityPLUS Reaction Buffer, with 7.5 mM MgCl2 5x concentrated

  • Expand High FidelityPLUS Reaction Buffer, without MgCl2 5x concentrated

  • MgCl2 Stock Solution 25 mM

  • PCR Nucleotide Mix

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash


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J R Brouwer et al.
Experimental cell research, 313(2), 244-253 (2006-12-08)
The human FMR1 gene contains a CGG repeat in its 5' untranslated region. The repeat length in the normal population is polymorphic (5-55 CGG repeats). Lengths beyond 200 CGGs (full mutation) result in the absence of the FMR1 gene product
Ken Kwok-Keung Chan et al.
Stem cells and development, 17(4), 825-836 (2008-09-16)
The utilization of human embryonic stem cells (hESC) in regenerative medicine largely depends on the development of technologies that will allow efficient genetic manipulation of the cells in vitro. Although a few studies have described the transfection of hESC for
Vici Varghese et al.
PloS one, 5(6), e10992-e10992 (2010-06-12)
The HIV-1 nucleoside RT inhibitor (NRTI)-resistance mutation, K65R confers intermediate to high-level resistance to the NRTIs abacavir, didanosine, emtricitabine, lamivudine, and tenofovir; and low-level resistance to stavudine. Several lines of evidence suggest that K65R is more common in HIV-1 subtype

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