MilliporeSigma
All Photos(2)

Documents

EHIFIP-RO

Roche

Expand High FidelityPLUS PCR System

sufficient for ≤500 reactions (03300226001), sufficient for ≤2,500 reactions (03300234001), suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included: no

Sign Into View Organizational & Contract Pricing

Synonym(s):
high fidelity pcr plus system, expand
Enzyme Commission number:

usage

sufficient for ≤2,500 reactions (03300234001)
sufficient for 2500 reactions
sufficient for ≤500 reactions (03300226001)
sufficient for 500 reactions

Quality Level

feature

High Fidelity PCR
dNTPs included: no
hotstart: no

packaging

pkg of 2,500 U (03300234001 [10x250 U])
pkg of 500 U (03300226001 [2x250 U])

manufacturer/tradename

Roche

parameter

72 °C optimum reaction temp.

technique(s)

PCR: suitable

input

purified DNA

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
EHIFIPN-ROEHIFI-ROEHIFIDNTP-RO
usage

sufficient for ≤2,500 reactions (03300234001)

usage

sufficient for ≤125 reactions (04743725001), sufficient for ≤500 reactions (04743733001)

usage

sufficient for ≤1,000 reactions (11759078001), sufficient for ≤200 reactions (11732650001), sufficient for ≤40 reactions (11732641001)

usage

sufficient for ≤1,000 reactions (04738276001), sufficient for ≤200 reactions (04738268001), sufficient for ≤40 reactions (04738250001)

feature

High Fidelity PCR

feature

High Fidelity PCR, dNTPs included, hotstart: no

feature

-

feature

High Fidelity PCR, dNTPs included, hotstart: no

packaging

pkg of 2,500 U (03300234001 [10x250 U])

packaging

pkg of 125 U (04743725001), pkg of 500 U (04743733001 [2x250 U])

packaging

pkg of 2,500 U (11759078001 [10x250 U]), pkg of 100 U (11732641001), pkg of 500 U (11732650001 [2x250 U])

packaging

pkg of 2,500 U (04738276001 [10x250 U]), pkg of 100 U (04738250001), pkg of 500 U (04738268001 [2x250 U])

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

parameter

72 °C optimum reaction temp.

parameter

72 °C optimum reaction temp.

parameter

-

parameter

-

General description

The Expand High FidelityPLUS PCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity.
The Expand High FidelityPLUS PCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone.
Expand High FidelityPLUS PCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets.

Application

The Expand High FidelityPLUS PCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This kit is the product of choice for the development of a new amplification assay or the optimization of an existing assay.
Use Expand High FidelityPLUS PCR System for:
  • PCR
  • RT-PCR
  • Primer extension
  • Carryover prevention

Features and Benefits

  • Safeguard against cross-contamination.
This is the choice for large numbers of amplifications.
  • Obtain sixfold higher fidelity and higher yields,
compared to Taq DNA Polymerase.
  • Efficiently label DNA.
Use this kit to label DNA with radioactive or nonradioactive nucleotides.

Packaging

1 kit containing 4 components

Quality

Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.

Unit Definition

Volume Activity: 5 U/μL

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Transition-metal-catalyzed cross-coupling is an efficient tool for constructing C-C, C-N and C-O bonds in organic synthesis. Intensive interest has been focused on the development of the most effective ligands that improve catalyst performance. Ligands play a key role in stabilizing and activating the central metal atom and fine-tuning the selectivity of transformation. Phosphines have proven to be the most significant class of ligands for cross couplings, such as Negishi, Suzuki, Stille, Heck, Sonogashira, and Buchwald-Hartwig cross-coupling.
Expand is a trademark of Roche

Kit Components Only

Product No.
Description

  • Expand High FidelityPLUS Enzyme Blend 5 U/μl

  • Expand High FidelityPLUS Reaction Buffer, with 7.5 mM MgCl2 5x concentrated

  • Expand High FidelityPLUS Reaction Buffer, without MgCl2 5x concentrated

  • MgCl2 Stock Solution 25 mM

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 7

1 of 7

GC-RICH PCR System sufficient for ≤50 reactions, pkg of 100 U, optimum reaction temp. 72 °C

Roche

12140306001

GC-RICH PCR System

Pwo DNA Polymerase

Roche

PWOPOL-RO

Pwo DNA Polymerase

Pwo Master sufficient for ≤100 reactions, pkg of 2.5 mL (10 x 250 μl)

Roche

03789403001

Pwo Master

PCR Nucleotide Mix solution, sufficient for 500 reactions (11581295001), sufficient for 5,000 reactions (11814362001), sufficient for 2,500 reactions (04638956001)

Roche

DNTPM-RO

PCR Nucleotide Mix

Uracil-DNA Glycosylase recombinant from E. coli K 12

Roche

11444646001

Uracil-DNA Glycosylase

Romee Van der Zee et al.
Environmental microbiology reports, 6(4), 401-413 (2014-07-06)
Infection of honeybees by the microsporidian Nosema ceranae is considered to be one of the factors underlying the increased colony losses and decreased honey production seen in recent years. However, these effects appear to differ in function of the climatic
Sabina Barresi et al.
PloS one, 9(3), e91351-e91351 (2014-03-19)
Oligophrenin-1 (OPHN1) encodes for a Rho-GTPase-activating protein, important for dendritic morphogenesis and synaptic function. Mutations in this gene have been identified in patients with X-linked intellectual disability associated with cerebellar hypoplasia. ADAR enzymes are responsible for A-to-I RNA editing, an

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service