EHIFIP-RO

Expand^™ High Fidelity^PLUS PCR System

sufficient for ≤500 reactions (03300226001), sufficient for ≤2,500 reactions (03300234001), suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included: no

• Synonym(s): high fidelity pcr plus system, expand
• EC Number: 2.7.7.7 (BRENDA, IUBMB)
• UNSPSC Code: 41106300
• NACRES: NA.21

Properties

• usage: sufficient for ≤2,500 reactions (03300234001); sufficient for 2500 reactions; sufficient for ≤500 reactions (03300226001); sufficient for 500 reactions
• Quality Level: 100
• feature: High Fidelity PCR; dNTPs included: no; hotstart: no
• packaging: pkg of 2,500 U (03300234001 [10x250 U]); pkg of 500 U (03300226001 [2x250 U])
• manufacturer/tradename: Roche
• parameter: 72 °C optimum reaction temp.
• technique(s): PCR: suitable
• input: purified DNA
• storage temp.: −20°C

Description

General description

The Expand High Fidelity^PLUS PCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity.
The Expand High Fidelity^PLUS PCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone.
Expand High Fidelity^PLUS PCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets.

Application

The Expand^™ High Fidelity^PLUS PCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This kit is the product of choice for the development of a new amplification assay or the optimization of an existing assay.
Use Expand High FidelityPLUS PCR System for:

• PCR[1]
• RT-PCR[2]
• Primer extension
• Carryover prevention

Features and Benefits

• Safeguard against cross-contamination.

This is the choice for large numbers of amplifications.

• Obtain sixfold higher fidelity and higher yields,

compared to Taq DNA Polymerase.

• Efficiently label DNA.

Use this kit to label DNA with radioactive or nonradioactive nucleotides.

Packaging

1 kit containing 4 components

Analysis Note

Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Volume Activity: 5 U/μL

Legal Information

Transition-metal-catalyzed cross-coupling is an efficient tool for constructing C-C, C-N and C-O bonds in organic synthesis. Intensive interest has been focused on the development of the most effective ligands that improve catalyst performance. Ligands play a key role in stabilizing and activating the central metal atom and fine-tuning the selectivity of transformation. Phosphines have proven to be the most significant class of ligands for cross couplings, such as Negishi, Suzuki, Stille, Heck, Sonogashira, and Buchwald-Hartwig cross-coupling.

Expand is a trademark of Roche

Kit Components Only

• Expand High Fidelity^PLUS Enzyme Blend 5 U/μl
• Expand High Fidelity^PLUS Reaction Buffer, with 7.5 mM MgCl₂ 5x concentrated
• Expand High Fidelity^PLUS Reaction Buffer, without MgCl₂ 5x concentrated
• MgCl₂ Stock Solution 25 mM

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: does not flash
• flash_point_c: does not flash