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ENDOGLUS-RO

Roche

Endoproteinase Glu-C Sequencing Grade

from Staphylococcus aureus V8

Synonym(s):

protease

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50 μG

$251.00

3 X 50 μG

$609.00

$251.00

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About This Item

UNSPSC Code:
12352204
EC Number:
3.4.21.19 (BRENDA, IUBMB)
NACRES:
NA.54
Specific activity:
≥15 units/mg protein
Biological source:
Staphylococcus aureus (V8)

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biological source

Staphylococcus aureus (V8)

form

lyophilized (salt-free), lyophilized

specific activity

≥15 units/mg protein

mol wt

Mr 27 kDa

packaging

pkg of 3 × 50 μg (11047817001), pkg of 50 μg (11420399001)

manufacturer/tradename

Roche

optimum pH

4.0-7.8

storage temp.

2-8°C

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This Item
ENDOLYSS-RO32471310791156001
Endoproteinase Glu-C Sequencing Grade from Staphylococcus aureus V8

Roche

ENDOGLUS-RO

Endoproteinase Glu-C Sequencing Grade

Endoproteinase Lys-C Sequencing Grade from Lysobacter enzymogenes

Roche

ENDOLYSS-RO

Endoproteinase Lys-C Sequencing Grade

Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus

Sigma-Aldrich

324713

Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus

Endoproteinase Glu-C (V8 Protease) from Staphylococcus aureus V8

Roche

10791156001

Endoproteinase Glu-C (V8 Protease)

specific activity

≥15 units/mg protein

specific activity

≥200 units/mg protein (at 25 °C, with Chromozym PL)

specific activity

≥10 units/mg protein

specific activity

20 U/mg

biological source

Staphylococcus aureus (V8)

biological source

bacterial (Lysobacter enzymogenes)

biological source

Staphylococcus aureus

biological source

-

form

lyophilized (salt-free)

form

lyophilized

form

lyophilized

form

lyophilized (salt-free)

mol wt

Mr 27 kDa

mol wt

33 kDa by SDS-PAGE (reducing)

mol wt

-

mol wt

30 kDa

optimum pH

4.0-7.8

optimum pH

8.5-8.8

optimum pH

-

optimum pH

8.0-8.5

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

General description

Endoproteinase Glu-C Sequencing Grade is a serine protease isolated from Staphylococcus aureus V8 as a highly purified and specific protease. Inhibitors of Endoproteinase Glu-C are DFP, α2-macroglobulin and TLCK.

Application

Use Endoproteinase Glu-C Sequencing Grade for protein structure analysis and for sequence analysis.[1][2]

Biochem/physiol Actions

Serine protease that very specifically hydrolyzes peptide bonds at the carboxylic side of Glu (in ammonium bicarbonate, pH 7.8, or ammonium acetate buffer, pH 4.0), or Glu and Asp (in phosphate buffer, pH 7.8). The specificity of Endoproteinase Glu-C Sequencing Grade is verified with the oxidized B-chain of insulin (insulin Box) as substrate. High concentrations of Endoproteinase Glu-C Sequencing Grade (1 part by weight enzyme with 10 parts by weight insulin Box) are incubated to detect traces of impurities, for example, contaminating proteases.
Heat inactivation: Most proteases sequencing grade are stopped by boiling for 5 minutes.

Features and Benefits

Free of impurities that may interfere with separation of peptides in RP HPLC.

Preparation Note

Working concentration: 1/100 to 1/20 (w/w)
With a minimum amount of 1 to 5 μg insulin Box, sufficient levels can be detected using the Column Aquapore RUP 300, 4.6 x 100 nm, 7 μm.
Working solution: Solvent is recommended in double-distilled water.
Solvents other than water have not been tested; Roche recommends using 50 mM Hepes, pH 7.8.
Storage conditions (working solution): +2°C to +8°C for 1-2 days.
Stability: At +2 to +8°C within specification range for 12 months; stored dry.
Lyophilized Endoproteinase Glu-C sequencing grade is reconstituted in double-dist. water. To avoid autolysis, the incubation temperature should not exceed 25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

Exclamation markHealth hazard
GHS07,GHS08

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
71853621
62542121
57696420
53511822
53511821

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Chen Li et al.
mAbs, 5(4), 565-575 (2013-06-12)
In developing biosimilar or biobetter products, comparability to the reference product is required to claim similar integrity or intended purpose. In this work, an anti-CD20 monoclonal antibody developed using RNA interference to decrease core fucosylation (RNAi-mediated) was comprehensively characterized by
Daniel Kolarich et al.
Nature protocols, 7(7), 1285-1298 (2012-06-09)
The comprehensive analysis of protein glycosylation is a major requirement for understanding glycoprotein function in biological systems, and is a prerequisite for producing recombinant glycoprotein therapeutics. This protocol describes workflows for the characterization of glycopeptides and their site-specific heterogeneity, showing

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