ERT-RO

Roche

Expand Reverse Transcriptase

Synonym(s):
reverse transcriptase, expand
Enzyme Commission number:

Quality Level

recombinant

expressed in E. coli (AP401 (k))

form

solution

packaging

pkg of 1,000 U (11785826001 [20 μl])
pkg of 5,000 U (11785834001 [100 μl])

manufacturer/tradename

Roche

parameter

42-43 °C optimum reaction temp.

application(s)

RT-PCR: suitable
RT-qPCR: suitable

input

purified RNA

detection method

probe-based

storage temp.

−20°C

General description

Expand Reverse Transcriptase is a RNA directed DNA polymerase optimized for amplification of cDNA fragments up to 13.5 kb in a two-step RT-PCR assay. The enzyme is a genetically engineered version of the Moloney Murine Leukemia Virus reverse transcriptase (M-MuLV-RT). A point mutation within the RNase H sequence reduces RNase H activity to undetectable levels. Detectable improvements are obtained with respect to higher amounts of full-length cDNA transcripts and longer transcripts compared to using the native M-MuLV-RT.

Specificity

Heat inactivation: 95 °C for 2 min
Reverse Transcriptase enzyme for RT-PCR reactions up to 3 kb

Application

Expand Reverse Transcriptase has been used in real-time PCR analysis for conversion of isolated RNA into cDNA. It has also been used in reverse transcription (RT). Expand Reverse Transcriptase may also be used for analyzing the sequence of the cDNA and comparing it to the genomic sequence. Important information can be obtained on intervening sequences (introns) and genomic recombinations.

Features and Benefits

  • Obtain higher amounts of full-length cDNA transcripts and longer transcripts up to 13.5kb
  • No RNase H activity
  • Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
  • Ideal for two-Step RT-PCR assay
  • Purification of cDNA before PCR reaction is not necessary

Packaging

1 set containing 3 components

Unit Definition

One unit is the enzyme activity that incorporates 1.0 nmol TMP into acid insoluble products in 10 min at +37 °C with poly(A)+ × (dT)15 as substrate.

Volume Activity: 50 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Expand is a trademark of Roche

Kit Components Only

Product No.
Description

  • Expand Reverse Transcriptase 50 U/μl

  • Expand Reverse Transcriptase Buffer 5x concentrated

  • DTT Stock Solution 100 mM

RIDADR

NONH for all modes of transport

Certificate of Analysis

Certificate of Origin

Edwin Yunhao Gong et al.
Methods in molecular biology (Clifton, N.J.), 1030, 195-203 (2013-07-04)
We have developed a duplex real-time RT-PCR assay for profiling antiviral inhibitors of four dengue virus (DENV) serotypes. In this assay, the primers and the probe for amplifying DENV were designed in the conserved regions of the genome after aligned...
M Hatzfeld et al.
The Journal of cell biology, 149(1), 209-222 (2000-04-04)
Plakophilin 1, a member of the armadillo multigene family, is a protein with dual localization in the nucleus and in desmosomes. To elucidate its role in desmosome assembly and regulation, we have analyzed its localization and binding partners in vivo....
Kathrin Ikels et al.
PloS one, 9(8), e105349-e105349 (2014-08-22)
The metabolic syndrome is a worldwide problem mainly caused by obesity. FTO was found to be a obesity-risk gene in humans and FTO deficiency in mice led to reduction in adipose tissue. Thus, FTO is an important factor for the...
André Spychala et al.
PloS one, 14(2), e0211937-e0211937 (2019-02-08)
Initially, the function of the fat mass and obesity associated (Fto) gene seemed to be primarily the regulation of the body weight. Here we show that loss of Fto results in a hyperactivation of the hypothalamic-pituitary-adrenal (HPA) axis. In consequence...
Christoph Gerhardt et al.
PloS one, 8(2), e57545-e57545 (2013-03-08)
Ventricular septal defects (VSDs) are the most common congenital heart defects in humans. Despite several studies of the molecular mechanisms involved in ventricular septum (VS) development, very little is known about VS-forming signaling. We observed perimembranous and muscular VSDs in...
Related Content
RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.
Read More

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