MilliporeSigma
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G418-RO

Roche

G-418 Solution

solution, =98% (TLC), suitable for transfection

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Synonym(s):
G 418 disulfate salt solution, O-2-Amino-2,7-dideoxy-Δ-glycero-α-Δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-Δ-streptamine, disulfate salt, neomycin, Antibiotic G418
Empirical Formula (Hill Notation):
C20H40N4O10 · 2H2SO4
CAS Number:
Molecular Weight:
692.71
MDL number:
PubChem Substance ID:

Quality Level

Assay

98% (TLC)

form

solution

mol wt

692.7 Da

packaging

pkg of 100 ml (04727894001 [5 x 20 ml, equivalent to 5 g])
pkg of 20 mL (04727878001 [equivalent to 1 g])

manufacturer/tradename

Roche

concentration

50 mg/mL

technique(s)

transfection: suitable

antibiotic activity spectrum

protozoa

Mode of action

protein synthesis | inhibits

storage temp.

2-8°C

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

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This Item
A1720G1279G5013
G-418 Solution solution, =98% (TLC), suitable for transfection

Roche

G418-RO

G-418 Solution

G 418 disulfate salt powder, BioReagent, suitable for cell culture

Sigma-Aldrich

A1720

G 418 disulfate salt

G 418 disulfate salt suitable for plant cell culture, BioReagent

Sigma-Aldrich

G1279

G 418 disulfate salt

G 418 disulfate salt potency: ≥720 μg per mg (dried basis)

Sigma-Aldrich

G5013

G 418 disulfate salt

form

solution

form

powder

form

powder

form

powder

concentration

50 mg/mL

concentration

-

concentration

-

concentration

-

technique(s)

transfection: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | plant: suitable

technique(s)

-

antibiotic activity spectrum

protozoa

antibiotic activity spectrum

Gram-negative bacteria

antibiotic activity spectrum

Gram-negative bacteria

antibiotic activity spectrum

Gram-negative bacteria

mode of action

protein synthesis | inhibits

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

General description

O-2-Amino-2,7-dideoxy-δ-glycero-α-δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-δ-streptamine, disulfate salt G-418, an aminoglycoside antibiotic, is used as a dominant selection agent in transfection experiments. The antibiotic interferes with the function of 80S ribosomes and blocks protein synthesis in eukaryotic cells. Transfection of neomycin-resistance genes into cells results in resistance to G-418 and enables the cells to grow in culture media containing G-418.

Application

G-418 Solution is used to select eukaryotic cells that are stably transfected with the neomycin resistance gene (neo), and to maintain the phenotype (neor) of resistant cells.
G-418 is also used to eliminate contaminating fibroblasts from mixed cultures.

Biochem/physiol Actions

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.

Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.

Features and Benefits

  • Convenient sterile-filtered solution for immediate use – saves valuable time and effort, and avoids potential errors arising from the preparation of sterile working stock solution from G-418 powder.
  • Consistent quality and potency – shows no lot-to-lot variation in its ability to select resistant cells.
  • Function tested in a cell culture assay - ensures effectiveness.
  • Very pure, as indicated by TLC and microbiological assays – allows you to use less antibiotic while producing greater selection pressure. Lack of contaminants means minimal toxicity.
  • Cost effective, since the selected colonies are stable.
Contents
Aqueous solution of G-418, 892 μg/mg, filtered through 0.2μm pore size membrane

Quality

Purity: = 98%, as shown by TLC.
Function testing: in cell culture.

Preparation Note

Working concentration: The optimal concentration of G-418 must be determined experimentally and varies with the cell type used.
Active weight: >700μg/mg
Recommended concentration in cell culture: The optimal concentration of G-418 must be determined experimentally; it varies from 100μg/ml to 1mg/ml, depending on the cell type used.
Specific rotation: 104° to 121°, in water (on dry basis, according to USP XXII).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available


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