G6PDHII-RO

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

grade II, from yeast

• Synonym(s): G6P-DH, Glucose-6-Phosephate Dehydrogenase
• UNSPSC Code: 12352204
• EC Number: 1.1.1.49 (BRENDA, IUBMB)
• NACRES: NA.54
• Specific activity: ~140 units/mg protein (At 25 °C with glucose-6-P as the substrate; standardized with BSA.)
• Biological source: yeast

Properties

• biological source: yeast
• form: suspension
• specific activity: ~140 units/mg protein (At 25 °C with glucose-6-P as the substrate; standardized with BSA.)
• packaging: pkg of 2 mL (10127671001 [5 mg/mL]), pkg of 5 mL (10737232001 [5 mg/mL])
• manufacturer/tradename: Roche
• optimum pH: 9.2
• pH range: 5-11
• shipped in: wet ice

Description

General description

Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.

Application

Glucose-6-Phosphate Dehydrogenase (G6P-DH) is a component of cofactor recycling systems for NADPH. Additional reagent glucose-6-phosphate.

It was used in enzymatic determination of C6 phosphorylation of glycogen.[1]

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6.0

Preparation Note

Activator: Mg²⁺ (5 to 10 mM)

Analysis Note

Contaminants: <0.001% CK, <0.05% GR, <0.01% 6-PGDH and PGluM each, <0.02% HK, <0.002% PGI

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash