HIL2-RO

Roche

Interleukin-2, human (hIL-2)

recombinant (E. coli)

Quality Level

biological source

human

recombinant

expressed in E. coli

assay

95% (SDS-PAGE)

form

solution

potency

>2 x 10^6 units/mg EC50

mol wt

(15,000 Da)

packaging

pkg of 10,000 U (10799068001 [5 μg, 50 ml])
pkg of 10,000 U (11011456001 [5 μg, 1 ml])
pkg of 50,000 U (11147528001 [25 μg, 5 ml])

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

concentration

10-20U/ml

impurities

<0.1 EU/μg

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... IL2(3558)

General description

Recombinant Interleukin-2, human (hIL-2) is produced in E. coli and purified by standard chromatographic techniques. Solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane.

Specificity

Species specificity: Recombinant IL-2, human is effective on mouse and human cells.

Application

Recombinant IL-2, human, allows:
  • The cultivation of human and murine IL-2 dependent T-cell lines and natural killer cell lines.
  • The proliferation of mitogen-activated T-lymphocytes and natural killer cells.
  • The establishment of human and murine thymocyte, splenocyte, or peripheral blood lymphocyte (PBL) derived T-cell lines.
  • The generation of human and murine lymphokine-activated killer (LAK) cells (1-11).
  • In vitro re-stimulation of lung cells obtained from mice.

Biochem/physiol Actions

Interlekin-2 (IL-2) is a potent pro-inflammatory cytokine. It plays a protective role in autoimmune chronic inflammation, when present in low amounts. It is involved in maintaining immune homeostasis where it controls the cross-talk between T regulatory and T effector cells. Studies in acute myelogenous leukemia (AML) mice models show that antibody based delivery of IL-2 to targets present in tumor-linked vasculature results in antileukemic activity.

Quality

Purity: >95% pure as determined by SDS-PAGE
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(1 EU corresponds to 0.1 ng)

Sequence

Chain Length 134 AA
The primary structure of recombinant, IL-2, human is identical to that of natural, human IL-2 (one polypeptide chain, 133 amino acids), however, recombinant IL-2 has an extra methionine at the amino-terminus (one polypeptide chain, 134 amino acids) and is not glycosylated. Glycosylation is not essential for biological activity.

Unit Definition

EC50 definition: The amount of hIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals ≤0.5 ng).

Unit Conversion: 1 BM unit = 3.25 NBSB units (natural IL-2)1 BM unit = 3.25 NBSB units (natural IL-2)

Physical form

Solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane

Preparation Note

Working concentration: 10 -20 U/ml
Established IL-2-dependent T-cell lines usually require 10-20 U/ml. Add IL-2 to the freezing medium for IL-2 dependent cell lines.

Working solution: Dilute the concentrated IL-2 solution (200 U/ml or 10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA or HSA (0.1%) or 1 to 10% serum.

Storage conditions (working solution): -15 to -25 °C
It is recommended to store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Specific activity/EC 50 : >2 x 106 U/mg <0.5 ng/ml (hIL-2, NIBSC, 1st international standard, 86/504), at least the same specific activity (EC50 ) compared to the indicated standard is guaranteed (19, 20). Human, recombinant IL-2 has the same biological activity in vitro as compared to human, natural IL-2 (15, 1–4).

Recommended Method of Dilution: Dilute the concentrated IL-2 solution (200 U/ml or 10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA or HSA (0.1%) or 1–10% serum.
Interleukin-2 (IL-2, also known as T-Cell Growth Factor, TCGF) is a lymphokine which is produced by lectin- or antigen-activated T-cells and plays an important immunoregulatory role. This factor, or lymphokine, was first identified by its ability to promote the long-term in vitro proliferation of activated T cells. It also promotes the generation and proliferation of cytotoxic T-cells, natural killer (NK) cells, and lymphokine- activated killer (LAK) cells (1–11). Recombinant human IL-2 allows the cultivation of human and murine IL-2-dependent T-cell lines and natural killer cell lines, the proliferation of mitogenactivated T-lymphocytes and natural killer cells, the establishment of human and murine thymocyte-, splenocyte-, or peripheral blood lymphocyte (PBL)- derived T-cell lines, and the generation of human and murine lymphokine-activated killer (LAK) cells (1-11). Established IL-2-dependant T-cell lines usually require 10-20 U/ml. Add IL-2 to the freezing medium for IL-2 dependant cell lines.

Other Notes

For life science research only. Not for use in diagnostic procedures.

RIDADR

NONH for all modes of transport

Certificate of Analysis
Certificate of Origin
Christoph Schliemann et al.
Cancer immunology research, 3(5), 547-556 (2015-02-13)
The antibody-based delivery of IL2 to extracellular targets expressed in the easily accessible tumor-associated vasculature has shown potent antileukemic activity in xenograft and immunocompetent murine models of acute myelogenous leukemia (AML), especially in combination with cytarabine. Here, we report our...
Marcel A Schijf et al.
Journal of virology, 86(21), 11472-11482 (2012-08-17)
Breast feeding reduces the risk of developing severe respiratory syncytial virus (RSV) infections in infants. In addition to maternal antibodies, other immune-modulating factors in human milk contribute to this protection. Specific dietary prebiotic oligosaccharides, similar to oligosaccharides present in human...
Elisa Lupino et al.
Immunology, 131(2), 231-241 (2010-05-15)
Stimulation of naïve CD4(+) T cells through engagement of the T-cell receptor (TCR) and the CD28 co-receptor initiates cell proliferation which critically depends on interleukin (IL)-2 secretion and subsequent autocrine signalling via the IL-2 receptor. However, several studies indicate that...
Koen Bartholomeeusen et al.
The Journal of biological chemistry, 288(20), 14400-14407 (2013-03-30)
Numerous studies have looked at the effects of histone deacetylase inhibitors (HDACis) on HIV reactivation in established transformed cell lines and primary CD4(+) T cells. However, their findings remain confusing, and differences between effects of class I- and class II-specific...
Agata Kosmaczewska
International journal of molecular sciences, 15(10), 18574-18592 (2014-10-17)
For many years, the role of interleukin-2 (IL-2) in autoimmune responses was established as a cytokine possessing strong pro-inflammatory activity. Studies of the past few years have changed our knowledge on IL-2 in autoimmune chronic inflammation, suggesting its protective role...

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