KAPA HotStart Mouse Genotyping Kit

Pricing and availability is not currently available.


sufficient for 100 reactions (KK7351)
sufficient for 500 reactions (KK7352)

Quality Level

shelf life

≤18 mo.

mfr. no.


shipped in

dry ice

storage temp.


General description

KAPA HotStart® Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of polymerase chain reaction (PCR)-ready DNA from mouse tissue in less than 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour, as compared to ≥1 day with conventional protocols. KAPA HotStart Mouse Genotyping Kit is designed for rapid extraction and amplification of DNA from mouse tissue.


KAPA HotStart® Mouse Genotyping Kit has been used for:
  • extraction and amplification from mouse tail, ear, and toe tissue
  • extraction and amplification of DNA from other animal tissue

Features and Benefits

Increased throughput, turnaround time and reliability
  • PCR-ready DNA generated in as little as 15 minutes.
  • Minimal handling, thereby reducing the risk of sample loss or contamination.
  • Sufficient template for multiple assays; easily scaled to handle samples in a 96-well format.
  • Fast and reliable amplification of DNA fragments across a wide range of amplicon lengths and GC contents.

Outperforms crude extraction methods and commercial kits
  • Increased turnaround times
  • Increased specificity
  • Decreased cost

Quick Notes:
  • Extract PCR-ready DNA from mouse tail, ear, or toe tissue in 15 minutes.
  • Dilute DNA extract 10-fold prior to PCR in 10 mM Tris-HCl (pH 8.0–8.5), and use 1 μL of DNA per 25 μL reaction.
  • Extracts are stable at -20°C for >6 months.
  • KAPA2G Fast (HotStart) Genotyping Mix contains the engineered KAPA2G Fast DNA Polymerase for fast PCR, along with buffer, MgCl2 (1.5 mM at 1X), and dNTPs (0.2 mM each at 1X).
  • Loading dye for electrophoresis is included in the KAPA2G Fast Genotyping Mix, which allows direct loading of PCR products onto agarose gels for analysis.
  • Use 15 sec annealing time, with 15 sec extension time for amplicons <1 kb, or 15–30 sec/kb extension for longer amplicons.


All components contained with KAPA HotStart Mouse Genotyping Kits are subjected to stringent quality control tests, are free of contaminating exo and endonuclease activities and meet strict requirements with respect to DNA contamination.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days).

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Kit Components Only

Product No.

  • KAPA Express Extract Enzyme 1 U/µL

  • 10X KAPA Express Extract Buffer

  • 2X KAPA2G Fast HotStart® Genotyping Mix with dye


Health hazard

Signal Word


Hazard Statements


NONH for all modes of transport

WGK Germany


Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis
Certificate of Origin
Stefanie Zeitler et al.
Cells, 9(11) (2020-11-22)
: The acid sphingomyelinase (ASM)/ceramide system exhibits a crucial role in the pathology of major depressive disorder (MDD). ASM hydrolyzes the abundant membrane lipid sphingomyelin to ceramide that regulates the clustering of membrane proteins via microdomain and lipid raft organization....
5-HT2A receptor deficiency alters the metabolic and transcriptional, but not the behavioral, consequences of chronic unpredictable stress.
Jaggar M, et al.
Neurobiology of stress, 7, 89-102 (2017)
Discovery of a new path for red blood cell generation in the mouse embryo.
Pinheiro I, et al.
bioRxiv, 309054-309054 (2018)
Maria Saliakoura et al.
Oncogene, 39(14), 2948-2960 (2020-02-09)
Enhanced prostaglandin production promotes the development and progression of cancer. Prostaglandins are generated from arachidonic acid (AA) by the action of cyclooxygenase (COX) isoenzymes. However, how cancer cells are able to maintain an elevated supply of AA for prostaglandin production...
Namit Kumar et al.
Development (Cambridge, England), 146(5) (2019-02-13)
Lineage-restricted transcription factors, such as the intestine-specifying factor CDX2, often have dual requirements across developmental time. Embryonic loss of CDX2 triggers homeotic transformation of intestinal fate, whereas adult-onset loss compromises crucial physiological functions but preserves intestinal identity. It is unclear...
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.