LMDH-RO

L-Malate Dehydrogenase (L-MDH)

from pig heart (mitochondrial)

• Synonym(s): L-MDH
• Enzyme Commission number: 1.1.1.37 (BRENDA, IUBMB)

Properties

• Quality Level: 100
• form: solution; suspension
• specific activity: ~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)
• packaging: pkg of 1 mL (10127248001 solution); pkg of 1 mL (10127256001 suspension); pkg of 5 mL (10127914001suspension)
• manufacturer/tradename: Roche
• optimum pH: 7.4-7.5(reduction of oxaloacetate); 9.2-9.5(oxidation of malate)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

L-malate:NAD⁺ oxidoreductase
The L-malate dehydrogenase enzyme is a nuclear gene product that is synthesized with a 24-residue amino-terminal signal peptide. This peptide is proteolytically cleaved during the translocation of the enzyme to the mitochondrial matrix.

Application

The enzyme L-malate dehydrogenase from pig heart has been used to measure PEPCK (phosphoenolpyruvate carboxykinase) activity. The oxaloacetate, produced by PEPCK, is reduced to malate via the oxidation of NADH, which in turn is measured at 340 nm using a spectrophotometer. The enzyme has also been used to measure oxaloacetate by measuring the reduction in NADH spectroscopically at 340 nm.

Biochem/physiol Actions

The enzyme L-malate dehydrogenase from pig heart catalyzes the oxidation of L-malate to oxaloacetate. The enzyme is an NAD-dependent mitochondrial dehydrogenase that functions in the tricarboxylic acid cycle. It is a component of the malate-aspartate shuttle that transports reducing equivalents across the inner mitochondrial membrane in the form of malate.

Quality

Contaminants: <0.002% GOT, <0.01% fumarase and LDH, each luM each, <0.02% HK, <0.002% PGI

Physical form

Solution in 50% glycerol (v/v), pH approximately 7

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Activator: – phosphate
– arsenate
– Mg²⁺
– Zn²⁺

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): No data available
• Flash Point(C): No data available