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PK-RO

Roche

Pyruvate Kinase (PK)

from rabbit muscle

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Synonym(s):
PK
Enzyme Commission number:
UNSPSC Code:
12352204

biological source

rabbit muscle

Quality Level

form

suspension

specific activity

~200 units/mg protein (at 25 °C (500 U/mg at 37 °C) with PEP as the substrate.)

packaging

pkg of 1 mL (10128155001 [10 mg])
pkg of 10 mL (10128163001 [100 mg])

manufacturer/tradename

Roche

optimum pH

7.0-7.5

storage temp.

2-8°C

Related Categories

General description

ATP:pyruvate 2-O-phosphotransferase
Pyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn2+ and phosphoenolpyruvate.

Application

Pyruvate kinase has been used to measure ATPase activity and in the determination of adenylate concentration.

Biochem/physiol Actions

Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. Apart from this, the enzyme exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate.

Quality

Contaminants: <0.001% GK, <0.002% “NADH oxidase”, and ATPase, each, <0.01% enolase, LDH, and myokinase, each

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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