PWOSUP-RO
Roche
Pwo SuperYield DNA Polymerase
suitable for PCR, Difficult Templates/Specialty Enzymes PCR, hotstart: no, dNTPs included: no
Recommended Products
biological source
(pyrococcus woesei)
Quality Level
form
liquid
usage
sufficient for ≤200 reactions (04340850001)
sufficient for 200 reactions
sufficient for ≤40 reactions (04340868001)
sufficient for 40 reactions
specific activity
5 U/μL
mol wt
~ 90 kDa
feature
Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included: no
hotstart: no
packaging
pkg of 100 U (04340868001)
pkg of 500 U (04340850001 [2 x 250 U])
manufacturer/tradename
Roche
concentration
50 U/mL
parameter
72 °C optimum reaction temp.
technique(s)
PCR: suitable
color
colorless
input
purified DNA
pH
8.0 (39 °F)
solubility
water: miscible
suitability
suitable for PCR
NCBI accession no.
UniProt accession no.
application(s)
life science and biopharma
foreign activity
Endonuclease 30 units, none detected
Nicking activity 30 units, none detected
storage temp.
−20°C
Related Categories
General description
Pwo SuperYield DNA Polymerase exhibits increased thermal stability with a half life of greater than 2 hours at +100 °C compared to Taq DNA Polymerase with a half life of less than 5 min at this temperature.
The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR.
PCR products are blunt-ended directly useable for blunt-end ligation.
Using the magnesium-containing reaction buffer supplied, the final MgCl2 concentration is 1.5mM.
Specificity
Application
- Pwo Super Yield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with a newly optimized buffer system. Pwo SuperYield DNA Polymerase is used for the amplification of DNA with the intent to sequence the amplification product or to clone the product (e.g., for the expression of the gene product). The high fidelity of this enzyme makes it particularly suitable for: High fidelity PCR
- Site-directed mutagenesis
- Cloning
- Gene expression
- Study of allelic polymorphism in individual RNA transcripts
- Characterization of rare mutations in tissue
- Characterization of the allelic stage of single cells or single DNA molecules
Features and Benefits
- Higher yield and 18-fold higher fidelity
- High performance with difficult templates.
- Reduce working steps in cloning.
Packaging
Quality
Unit Definition
Unit Assay: Incubation buffer for assay on activated DNA
20 mM Tris-HCl, pH 8.8 (20 °C), 50 mM KCl, 2.5 mM MgCl2, 10 mM 2-mercaptoethanol, 0.2 mM of each dATP, dCTP, dGTP, dTTP.
Incubation procedure
12.5 mg activated calf thymus DNA and 0.1 mCi [α-32P]dCTP are incubated with 0.01 to 0.1 U Pwo SuperYield DNA Polymerase in 50 μl incubation buffer with a paraffin-oil overlay at +70 °C for 30 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation followed by scintillation counting.
Volume Activity: 5 U/μl
Other Notes
Pwo DNA Polymerase accepts modified nucleotides like digoxigenin-dUTP, biotin-dUTP, or fluorescein-dUTP. Thus, it can add these nucleotides to DNA during PCR. These nonradioactively labeled products can be used as a hybridization probe in many applications.
Legal Information
Kit Components Only
- Pwo SuperYield DNA Polymerase 5 U/μl
- PCR buffer, containing 15 mM MgSO4 10x concentrated
- GC-RICH Solution 5x concentrated
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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