MilliporeSigma
All Photos(2)

Documents

RDLIG-RO

Roche

Rapid DNA Dephos & Ligation Kit

sufficient for 40 reactions (04898117001), sufficient for 160 reactions (04898125001), optimum reaction temp. 37 °C

Sign Into View Organizational & Contract Pricing

Synonym(s):
cloning of dna fragments, dna ligase, ligases
Enzyme Commission number:

usage

sufficient for 160 reactions (04898125001)
sufficient for 40 reactions (04898117001)

Quality Level

manufacturer/tradename

Roche

parameter

37 °C optimum reaction temp.

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
11635379001LIG2LIG1
vibrant-m

RDLIG-RO

Rapid DNA Dephos & Ligation Kit

vibrant-m

11635379001

Rapid DNA Ligation Kit

vibrant-m

LIG2

QuickLink DNA Ligation Kit

vibrant-m

LIG1

DNA Ligation Kit

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

parameter

37 °C optimum reaction temp.

parameter

-

parameter

-

parameter

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

General description

The Rapid DNA Dephos & Ligation Kit enables fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments.

Specificity

Specificity: rAPid Alkaline Phosphatase
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rApid Alkaline Phosphatase:
Two minutes at 75 °C.
T4 DNA Ligase:
10 minutes at 65 °C.

Application

The Rapid DNA Dephos & Ligation Kit minimizes the time needed to perform diverse applications, including:
  • Cloning of fragments into either plasmid or phage vectors
  • Linker ligation
  • Recircularization of linear vector DNA
  • Generation of libraries

Packaging

1 kit containing 5 components

Unit Definition

Unit Definition: rAPid Alkaline Phosphatase
One unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes one μmol of 4-nitrophenyl phosphate in one minute at +37 °C under assay conditions.

Volume Activity: rApid Alkaline Phosphatase: 1 U/μl
T4 DNA Ligase: 5 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • rAPid Alkaline Phosphatase Buffer 10x concentrated

  • rAPid Alkaline Phosphatase 1 U/μl

  • DNA Dilution Buffer 5x concentrated

  • T4 DNA Ligation Buffer 2x concentrated

  • T4 DNA Ligase 5 U/μl

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 6

1 of 6

Francesca Colombo et al.
Molecular cancer, 10, 81-81 (2011-07-09)
The MFSD2A gene maps within a linkage disequilibrium block containing the MYCL1-EcoRI polymorphism associated with prognosis and survival in lung cancer patients. Survival discrepancies between Asians and Caucasians point to ethnic differences in allelic frequencies of the functional genetic variations.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service