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TAQL-RO

Roche

Taq DNA Polymerase, 1 U/μl

suitable for PCR, hotstart: no, dNTPs included: no

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Synonym(s):
dna amplification, pcr, polymerase, primer extension
Enzyme Commission number:

usage

sufficient for ≤2,000 reactions (11647687001)
sufficient for ≤500 reactions (11647679001)

Quality Level

feature

dNTPs included: no
hotstart: no

packaging

pkg of 1,000 U (11647687001 [4 x 250 U])
pkg of 250 U (11647679001)

manufacturer/tradename

Roche

parameter

72 °C optimum reaction temp.

technique(s)

PCR: suitable

input

purified DNA

optimum pH

~9.0 (20 °C)

storage temp.

−20°C

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This Item
FPCRN-ROD8045CORET
Taq DNA Polymerase, 1 U/μl suitable for PCR, hotstart: no, dNTPs included: no

Roche

TAQL-RO

Taq DNA Polymerase, 1 U/μl

technique(s)

PCR: suitable

technique(s)

-

technique(s)

PCR: suitable

technique(s)

PCR: suitable

optimum pH

~9.0 (20 °C)

optimum pH

-

optimum pH

-

optimum pH

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

usage

sufficient for ≤2,000 reactions (11647687001)

usage

sufficient for ≤1,250 reactions (04738403001), sufficient for ≤2,500 reactions (04738420001), sufficient for ≤250 reactions (04738357001), sufficient for ≤50 reactions (04738314001), sufficient for ≤500 reactions (04738381001)

usage

sufficient for 125 reactions, sufficient for 1500 reactions, sufficient for 500 reactions

usage

sufficient for 250 reactions

General description

The enzyme was cloned in E.coli and is isolated to be free of unspecific endo- or exonucleases according to the current quality control procedurese. Taq DNA Polymerase is a highly processive 5′–3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It consists of a single polypeptide chain with a molecular weight of approximately 95 kDa. The enzyme exhibits highest activity at a pH of around 9 (adjusted at 20 °C) and temperatures around +75 °C. Taq DNA Polymerase also accepts modified deoxyribonucleosidetriphosphates as substrates, and can be used to label DNA-fragments either with radionucleotides, digoxigenin, fluorescein or biotin.
The high processivity, absence of exonuclease activity and temperature optima of Taq DNA Polymerase enable the use of this enzyme in DNA sequencing, especially where the resolution of secondary structures plays a major role.

Application

This lower concentration of our recombinant Taq DNA Polymerase allows small amounts of the polymerase to be pipetted more accurately and conveniently. In all other respects, this preparation is identical to our higher concentration (5 U/μl) preparation and can be used for:
  • PCR
  • RT-PCR
  • Other primer-extension reactions, such as sequencing and labeling

Packaging

1 kit containing 4 components

Unit Definition

One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA within 60 min at +65 °C under the assay conditions stated above.

Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.

Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 1 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Kit Components Only

Product No.
Description

  • Taq DNA Polymerase 1 U/μl

  • PCR Buffer with MgCl<sub>2</sub> 10x concentrated

  • MgCl<sub>2</sub> Stock Solution

  • PCR Buffer without MgCl<sub>2</sub>

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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