MilliporeSigma
All Photos(3)

TAQ-RO

Roche

Taq DNA Polymerase, 5 U/μl

optimum pH ~9.0 (20 °C), optimum reaction temp. 72 °C

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
dna amplification, pcr, polymerase, pcr, primer extension, dna amplification, primer extension
Enzyme Commission number:

usage

sufficient for ≤1,000 reactions (11146173001)
sufficient for ≤10,000 reactions (11435094001)
sufficient for ≤2,000 reactions (11418432001)
sufficient for ≤5,000 reactions (11596594001)
sufficient for ≤200 reactions (11146165001)

Quality Level

packaging

pkg of 1,000 U (11418432001 [4 x 250 U])
pkg of 2,500 U (11596594001 [10 x 250 U])
pkg of 5,000 U (11435094001 [20 x 250 U])
pkg of 100 U (11146165001)
pkg of 500 U (11146173001 [1 x 500 U])

manufacturer/tradename

Roche

parameter

72 °C optimum reaction temp.

optimum pH

~9.0 (20 °C)

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
TAQL-RO11480022001D7442
Taq DNA Polymerase, 5 U/μl optimum pH ~9.0 (20 °C), optimum reaction temp. 72 °C

Roche

TAQ-RO

Taq DNA Polymerase, 5 U/μl

Taq DNA Polymerase, 1 U/μl suitable for PCR, hotstart: no, dNTPs included: no

Roche

TAQL-RO

Taq DNA Polymerase, 1 U/μl

Tth DNA Polymerase pkg of 500 U (2 x 250_U), sufficient for ≤200 reactions

Roche

11480022001

Tth DNA Polymerase

optimum pH

~9.0 (20 °C)

optimum pH

~9.0 (20 °C)

optimum pH

~9.0 (25 °C)

optimum pH

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

200

usage

sufficient for ≤1,000 reactions (11146173001)

usage

sufficient for ≤2,000 reactions (11647687001), sufficient for ≤500 reactions (11647679001)

usage

sufficient for ≤200 reactions

usage

sufficient for 1500 reactions, sufficient for 250 reactions

packaging

pkg of 1,000 U (11418432001 [4 x 250 U])

packaging

pkg of 1,000 U (11647687001 [4 x 250 U]), pkg of 250 U (11647679001)

packaging

pkg of 500 U (2 x 250_U)

packaging

-

General description

Taq DNA Polymerase is a highly processive 5′→3′ DNA polymerase that lacks 3′→5′ exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.
Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM, a strain lacking Taq I restriction endonuclease. The enzyme was cloned in E.coli.

Application

Taq DNA Polymerase can be used in simple, routine PCR. Roche Applied Science Taq DNA polymerase is held to rigorous purity and quality standards. This preparation of recombinant Taq DNA Polymerase can be applied for:
  • PCR
  • RT-PCR
  • qPCR
  • Other primer-extension reactions, such as sequencing and labeling

Features and Benefits

Reliable reproducible results:
High lot-to-lot consistency.
No need to test each lot:
Taq DNA Polymerase is rigorously tested.
Prevent PCR carryover:
dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination.

Packaging

1 kit containing 2 components

Quality

Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75°C and pH 9.
Lack of restriction endonuclease:
The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures.

Unit Definition

One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 60 min at +65 °C under the assay conditions given above.

Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.

Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 5 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Kit Components Only

Product No.
Description

  • Taq DNA Polymerase 5 U/μl

  • PCR Buffer with MgCl<sub>2</sub> 10x concentrated

  • MgCl<sub>2</sub> Stock Solution

  • PCR Buffer without MgCl<sub>2</sub>

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 6

1 of 6

H N Chinivasagam et al.
Journal of applied microbiology, 103(2), 418-426 (2007-07-26)
To investigate the occurrence and levels of Arcobacter spp. in pig effluent ponds and effluent-treated soil. A Most Probable Number (MPN) method was developed to assess the levels of Arcobacter spp. in seven pig effluent ponds and six effluent-treated soils
Mónica Alejandra Rosales-Reynoso et al.
Archives of medical research, 41(2), 110-118 (2010-05-18)
Although sporadic cases of cancer in patients with fragile X syndrome (FXS) have been reported, extensive studies carried out in Denmark and Finland concluded that cancer incidence in these patients is lower than in the general population. On the other
Pattabhiraman Shankaranarayanan et al.
Nature protocols, 7(2), 328-338 (2012-01-28)
Linear amplification of DNA (LinDA) by T7 polymerase is a versatile and robust method for generating sufficient amounts of DNA for genome-wide studies with minute amounts of cells. LinDA can be coupled to a great number of global profiling technologies.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service