11-0740

Formamide

Name: Formamide
Brand: SAJ

JIS special grade, ≥98.5%

Synonym(s):

Amide C₁, Formic amide

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About This Item

Linear Formula:

HCONH₂

CAS Number:

75-12-7

Molecular Weight:

45.04

Beilstein/REAXYS Number:

505995

EC Number:

200-842-0

MDL number:

MFCD00007941

UNSPSC Code:

12352111

PubChem Substance ID:

329749139

Grade:

JIS special grade

Assay:

≥98.5%

Technique(s):

hybridization: suitable

Bp:

210 °C (lit.)

Vapor pressure:

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

Key Documents

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grade

JIS special grade

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

assay

≥98.5%

form

liquid

autoignition temp.

932 °F

expl. lim.

2.7-19 % (lit.)

availability

available only in Japan

technique(s)

hybridization: suitable

refractive index

n20/D 1.447 (lit.)

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

density

1.134 g/mL at 25 °C (lit.)

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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Analytical Chemistry

Analytical Chromatography

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Show Differences

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This Item	11-0750	221198	47671
Sigma-Aldrich 11-0740 Formamide	Sigma-Aldrich 11-0750 Formamide	Sigma-Aldrich 221198 Formamide	Sigma-Aldrich 47671 Formamide
assay ≥98.5%	assay ≥98.5%	assay ≥99.5%	assay ≥99.5% (T)
technique(s) hybridization: suitable	technique(s) hybridization: suitable	technique(s) PCR: suitable	technique(s) FISH: suitable, hybridization: suitable, protein extraction: suitable
grade JIS special grade	grade SAJ first grade	grade ACS reagent	grade for molecular biology
form liquid	form liquid	form liquid	form liquid
mp 2-3 °C (lit.)	mp 2-3 °C (lit.)	mp 2-3 °C (lit.)	mp 2-3 °C (lit.)
refractive index n20/D 1.447 (lit.)	refractive index n20/D 1.447 (lit.)	refractive index n20/D 1.447 (lit.)	refractive index n20/D 1.447 (lit.)

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

pictograms

GHS08

signalword

Danger

hcodes

H351,H360D,H373

pcodes

P202 - P260 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

target_organs

Blood

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 1

flash_point_f

305.6 °F

flash_point_c

152 °C

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Technical variability is greater than biological variability in a microarray experiment but both are outweighed by changes induced by stimulation.

Penelope A Bryant et al.

PloS one, 6(5), e19556-e19556 (2011-06-10)

A central issue in the design of microarray-based analysis of global gene expression is that variability resulting from experimental processes may obscure changes resulting from the effect being investigated. This study quantified the variability in gene expression at each level

Single-tube nonaplex microsatellite PCR panel for preimplantation genetic diagnosis of Hb Bart's hydrops fetalis syndrome.

Min Chen et al.

Prenatal diagnosis, 35(6), 534-543 (2015-02-03)

To develop a single-tube multi-marker assay for improved preimplantation genetic diagnosis (PGD) of deletional and/or non-deletional Hb Bart's hydrops fetalis syndrome, providing haplotype confirmation of deletional status, and maximization of linkage informativity. We performed in silico mining to identify novel

Mutation-free baby born from a mitochondrial encephalopathy, lactic acidosis and stroke-like syndrome carrier after blastocyst trophectoderm preimplantation genetic diagnosis.

Björn Heindryckx et al.

Mitochondrion, 18, 12-17 (2014-08-28)

To investigate the applicability of preimplantation genetic diagnosis (PGD), we used trophectoderm (TE) biopsy to determine the mutation load in a 35-year-old female with mitochondrial encephalopathy, lactic acidosis and stroke-like syndrome (MELAS). Transfer of a mutation-free blastocyst gave birth to

Molecular Epidemiology of Mycoplasma pneumoniae: Genotyping Using Single Nucleotide Polymorphisms and SNaPshot Technology.

A Touati et al.

Journal of clinical microbiology, 53(10), 3182-3194 (2015-07-24)

Molecular typing of Mycoplasma pneumoniae is an important tool for identifying grouped cases and investigating outbreaks. In the present study, we developed a new genotyping method based on single nucleotide polymorphisms (SNPs) selected from the whole-genome sequencing of eight M.

From formamide to purine: an energetically viable mechanistic reaction pathway.

Jing Wang et al.

The journal of physical chemistry. B, 117(8), 2314-2320 (2013-01-26)

A step-by-step mechanistic pathway following the transformation of formamide to purine through a five-membered ring intermediate has been explored by density functional theory computations. The highlight of the mechanistic route detailed here is that the proposed pathway represents the simplest

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