00185

Acetamide Nutrient Broth

NutriSelect^® Basic, powder, selective by defined nutrients for Pseudomonas aeruginosa, pack of 500 g, suitable for microbiology

• UNSPSC Code: 41171607
• NACRES: NA.85
• MDL number: MFCD00239573
• Manufacturer/tradename: NutriSelect^® Basic
• Form: powder
• Suitability: selective by defined nutrients for Pseudomonas aeruginosa
• Application(s): bioburden testing; environmental; food and beverages; water monitoring; microbiology

Properties

• Product Name: Acetamide Nutrient Broth, NutriSelect^® Basic, powder, selective by defined nutrients for Pseudomonas aeruginosa, pack of 500 g, suitable for microbiology
• sterility: non-sterile
• product line: BioChemika
• form: powder
• Quality Level: 100
• shelf life: limited shelf life, expiry date on the label
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbe id | utilization test: suitable
• final pH: 7.0±0.2 (25 °C)
• application(s): bioburden testing; environmental; food and beverages; water monitoring; microbiology
• suitability: selective by defined nutrients for Pseudomonas aeruginosa

Description

Application

Synthetic medium to detect microbial utilization of acetamide. Corresponds to the suggestions of the "Deutsches Institut für Normung (DIN)" and the test procedures set out in §35 LMBG for the examination of water for Pseudomonas aeruginosa.

Other Notes

Composition

Part A (Cat. No. 62192-109.4-KC)
Magnesium sulfate 0.158g/L
Sodium chloride 0.2g/L
Sodium molybdate 0.005g/L
Ferrous sulfate 0.0005g/L
Dipotassium hydrogen phosphate 0.2g/L

Part B (Cat. No. 00160-390.6G-KC)
Acetamide 2.0g/L

Decomposition of acetamide as taxonomic marker for some species of the genus Pseudomonas[1]; Comparative studies on the enrichment of P. aeruginosa[2]

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Directions:
Dissolve 0.56 g Acetamide Nutrient Broth Part A and 2 g Acetamide in 950 ml distilled water. Check the pH and if necessary, adjust. Make up to 1 litre and pour into tubes. Autoclave at 121°C for 15 minutes.

Technique:
Medium is inoculated with a couple off loops from the culture to be assayed and it is incubated at 32-35°C for 24-48 hours before going to the isolation medium.
To confirm Ps. aeruginosa, inoculate a loop of culture in Asparagine Proline Broth (Cat. No. 17129) and incubate at 35-37°C for 24 hours. After this period, pour 1-2 drops of Nessler′s Reagent
(Cat. No. 72190) on to the culture and verify ammonia production: a change to yellow will mean ammonia production positive and therefore, Ps. aeruginosa presence.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08
• signalword: Warning
• hcodes: H351
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges