0120214

Buffered Peptone Water in Bags

For the serial dilution and pre-enrichment of bacteria, in particular pathogenic members of the Enterobacteriaceae, suitable for microbiology

• Synonym(s): BPW in bags, Peptone water (Buffered)

Properties

• Agency: ISO 6579; ISO 6887; according to GB 4789.30-2016; according to GB 4789.40-2016; according to ISO 21528-1:2017; according to ISO 22964:2017
• Quality Level: 200
• sterility: sterile
• form: liquid
• shelf life: limited shelf life, expiry date on the label
• feature: ready-to-use
• composition: casein peptone, 10 g/L ; di-sodium hydrogen phosphate 12H₂O, 9 g/L ; potassium dihydrogen phosphate, 1.5 g/L ; sodium chloride, 5 g/L
• technique(s): microbiological culture: suitable
• final pH: 7.0±0.2 (25 °C)
• application(s): environmental; food and beverages; industrial qc; pathogen testing; sample preparation; microbiology
• suitability: Citrobacter ssp.; Cronobacter ssp.; Escherichia coli; Klebsiella ssp.; Proteus ssp.; Providencia ssp.; Pseudomonas ssp.; Salmonella spp.; Shigella ssp.; Staphylococcus ssp.; Streptococcus ssp.; Yersinia ssp.; bacteria; coliforms; enterobacteriaceae

Description

General description

Buffered peptone water in bags is a pre-enrichment medium designed to help the recovery of sub-lethally damaged microorganisms before transferring to a selective medium. This pre-enrichment medium is free from inhibitors and is well buffered and provides conditions for resuscitation of the cells that have been injured by processes of food preservation. The media contains Peptone including an enzymatic digest of casein as a source of carbon, nitrogen, vitamins, and minerals. Sodium chloride maintains the osmotic balance and phosphates buffer the medium. The broth is rich in nutrients and produces high resuscitation rates for sub-lethally injured bacteria and supports intense growth.

Application

Buffered Peptone Water is used as :

• a non-selective pre-enrichment medium for the detection of Salmonella in food products and environmental specimens. It complies with the standard EN ISO 6579 (1) and amendment A1 (annex D) (8),
• a diluent for the enumeration of the micro-organisms. It complies with the standards EN ISO 6887 (2, 3, 4, 5) and 8261 (6),
• a diluent for the enumeration of Listeria monocytogenes. It complies with the standard EN ISO 11290-2 (7).

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable