MilliporeSigma
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01696

Sigma-Aldrich

Acrylamide

for Northern and Southern blotting, powder blend

Synonym(s):
2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide
Linear Formula:
CH2=CHCONH2
CAS Number:
Molecular Weight:
71.08
Beilstein:
605349
EC Number:
MDL number:
eCl@ss:
39031205
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

product line

BioReagent

Assay

≥99.5% (GC)

form

powder blend

technique(s)

electrophoresis: suitable

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

H2O: 0.25 g/mL at 20 °C, clear, colorless

storage temp.

2-8°C

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

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Acrylamide for Northern and Southern blotting, powder blend

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01696

Acrylamide

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Acrylamide certified reference material, TraceCERT®

Supelco

08267

Acrylamide

grade

for molecular biology

grade

-

grade

-

grade

certified reference material, TraceCERT®

form

powder blend

form

powder

form

crystals

form

-

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

≤0.005% Acrylic acid, ≤0.005% Insoluble in water

impurities

-

impurities

-

vapor density

2.45 (vs air)

vapor density

2.45 (vs air)

vapor density

2.45 (vs air)

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

General description

Acrylamide powder is a white, odorless, crystalline solid. It is commonly used in molecular biology labs as a cross linking agent to cast polyacrylamide gels for nucleic acid electrophoresis.

Application

Suitable for making polyacrylamide gels according to established recipes.

Caution

Acrylamide powder is highly toxic through inhalation and skin contact. Acrylamide is considered to be a probable carciongen in humans. Standard laboratory nitrile gloves and full sleeve lab coats should be worn when handling acrylamide in any form. Dust masks or working in a fume hood is advised when handling acrylamide powder.

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

280.4 °F - closed cup

Flash Point(C)

138 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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