01696

Acrylamide

for Northern and Southern blotting, powder blend

• Synonym(s): 2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide
• Linear Formula: CH₂=CHCONH₂
• CAS Number: 79-06-1
• Molecular Weight: 71.08
• Beilstein: 605349
• EC Number: 201-173-7
• MDL number: MFCD00008032
• eCl@ss: 39031205
• PubChem Substance ID: 329747346
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• vapor density: 2.45 (vs air)
• vapor pressure: 0.03 mmHg ( 40 °C)
• product line: BioReagent
• Assay: ≥99.5% (GC)
• form: powder blend
• technique(s): electrophoresis: suitable
• impurities: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• bp: 125 °C/25 mmHg (lit.)
• mp: 82-86 °C (lit.)
• solubility: H₂O: 0.25 g/mL at 20 °C, clear, colorless
• storage temp.: 2-8°C
• SMILES string: NC(=O)C=C
• InChI: 1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)
• InChI key: HRPVXLWXLXDGHG-UHFFFAOYSA-N

Description

General description

Acrylamide powder is a white, odorless, crystalline solid. It is commonly used in molecular biology labs as a cross linking agent to cast polyacrylamide gels for nucleic acid electrophoresis.

Application

Suitable for making polyacrylamide gels according to established recipes.

Caution

Acrylamide powder is highly toxic through inhalation and skin contact. Acrylamide is considered to be a probable carciongen in humans. Standard laboratory nitrile gloves and full sleeve lab coats should be worn when handling acrylamide in any form. Dust masks or working in a fume hood is advised when handling acrylamide powder.

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H301 - H312 + H332 - H315 - H317 - H319 - H340 - H350 - H361f - H372
• Precautionary Statements: P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• Target Organs: Peripheral nervous system
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): 280.4 °F - closed cup
• Flash Point(C): 138 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves