03609

Sigma-Aldrich

Ethylenediaminetetraacetic acid

BioUltra, ≥99.0% (KT)

Synonym(s):
Edathamil, (Ethylenedinitrilo)tetraacetic acid, EDTA, Ethylenedinitrilotetraacetic acid
Linear Formula:
(HO2CCH2)2NCH2CH2N(CH2CO2H)2
CAS Number:
Molecular Weight:
292.24
Beilstein/REAXYS Number:
1716295
EC Number:
MDL number:
eCl@ss:
39030907
PubChem Substance ID:
NACRES:
NA.25

product line

BioUltra

Quality Level

assay

≥99.0% (KT)

form

powder

impurities

insoluble matter, passes filter test

mp

250 °C (dec.) (lit.)

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤100 mg/kg

cation traces

Al: ≤10 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤500 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.1 M in 1 M NaOH

UV absorption

λ: 260 nm Amax: ≤1.00
λ: 280 nm Amax: ≤0.05

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

Ethylenediaminetetraacetic acid (EDTA) is a hexadentate ligand and chelating agent with the ability to sequester divalent cations such as calcium and cesium. EDTA is used in a wide range of applications where the sequestration of divalent cations is required or useful. EDTA is used in the study of artificial restriction DNA cutters.

Other Notes

Determination of anions and metal cations by single-column chromatography with EDTA as eluent using conductivity and UV detection ; Buffer component in DNA digest analysis with capillary electrophoresis

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Yuichiro Aiba et al.
Chemical Society reviews, 40(12), 5657-5668 (2011-05-14)
This tutorial review provides recent developments in artificial cutters for site-selective scission of DNA with the focus on chemistry-based DNA cutters. They are useful tools for molecular biology and biotechnology, since their site-selectivity of scission is much higher than that...
S. Matsushita
Journal of Chromatography A, 312, 327-327 (1984)
A Paulus et al.
Electrophoresis, 14(1-2), 27-35 (1993-01-01)
DNA digest analysis with polymer-filled capillaries in capillary electrophoresis is described. The samples analyzed consisted of commercially available standards including a 100 base pair ladder with repeating units up to 2000 base pairs. Three DNA digests covered the most common...
Des R Richardson et al.
Journal of medicinal chemistry, 49(22), 6510-6521 (2006-10-27)
There has been much interest in the development of iron (Fe) chelators for the treatment of cancer. We developed a series of di-2-pyridyl ketone thiosemicarbazone (HDpT) ligands which show marked and selective antitumor activity in vitro and in vivo. In...
Cecilia Lezcano et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 27(9), 1193-1202 (2014-01-18)
Of more than 150 000 published studies evaluating new biomarkers, fewer than 100 biomarkers have been implemented for patient care. One reason for this is lack of rigorous testing by the medical community to validate claims for biomarker clinical relevance, and...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.