03677

Ethylenediaminetetraacetic acid disodium salt dihydrate

BioUltra, for molecular biology, ≥99.0% (T)

• Synonym(s): Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na₂, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na₂
• Empirical Formula (Hill Notation): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
• CAS Number: 6381-92-6
• Molecular Weight: 372.24
• Beilstein: 3900609
• EC Number: 205-358-3
• PubChem Substance ID: 24845544
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• product line: BioUltra
• Assay: ≥99.0% (T)
• form: powder or crystals
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 4.0-5.5 (25 °C, 0.1 M in H₂O)
• mp: 248 °C (dec.) (lit.)
• solubility: H₂O: 0.1 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤20 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.1 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.145; λ: 280 nm A_max: 0.025
• SMILES string: O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
• InChI key: OVBJJZOQPCKUOR-UHFFFAOYSA-L

Description

Application

Ethylenediaminetetraacetic acid disodium salt dehydrate can be used to study molecular biology, biochemicals, solutions and reagents, biological buffers, DNA and RNA purification, inorganic salts, essential chemicals and chelators. Ethylenediaminetetraacetic acid disodium salt dehydrate has been used in a study to advance monitoring of illegal thyreostatic drugs in livestock. Ethylenediaminetetraacetic acid disodium salt dehydrate has also been used in a study for the determination of 232Th in seawater by ICP-MS after preconcentration and separation using a chelating resin.

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Other Notes

Running-buffer component for non-denaturing or urea-containing denaturing polyacrylamide gels in nucleic acid electrophoresis

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Warning
• Hazard Statements: H332 - H373 - H412
• Precautionary Statements: P260 - P271 - P273 - P304 + P340 + P312 - P314 - P501
• Hazard Classifications: Acute Tox. 4 Inhalation - Aquatic Chronic 3 - STOT RE 2 Inhalation
• Target Organs: Respiratory Tract
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves