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03777

Sigma-Aldrich

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

BioUltra, for molecular biology, ≥99.0% (T)

Synonym(s):
EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid
Linear Formula:
[-CH2OCH2CH2N(CH2CO2H)2]2
CAS Number:
Molecular Weight:
380.35
Beilstein:
1717370
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

assay

≥99.0% (T)

form

powder

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

ign. residue

≤0.05% (as SO4)

loss

≤1.0% loss on drying, 110 °C

mp

241 °C (dec.) (lit.)

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤200 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

absorption

cut-off at 263 nm in 1 M NaOH at 0.1 M

SMILES string

OC(=O)CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)

InChI key

DEFVIWRASFVYLL-UHFFFAOYSA-N

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Application

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid can be used to study molecular biology, biochemicals, reagents and chelators. Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used in a study that illustrated the response of the β-cyclodextrin-hemin complex in the induction of cucumber adventitious rooting through heme oxygenase-1-dependent mechanism and calcium signaling.
A chelating agent useful for the determination of calcium in the presence of magnesium.

Biochem/physiol Actions

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.

Other Notes

Buffer component in dideoxy sequencing of RNA using reverse transcriptase.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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