04551

Schaeffer and Fulton Spore Stain Kit

for microscopic observation of bacteria and endospores

• Synonym(s): Spore Stain Kit according to Schaeffer and Fulton
• UNSPSC Code: 41171621
• NACRES: NA.85
• eCl@ss: 32161000
• Technique(s): microbe id | staining: suitable

Properties

• product line: BioChemika
• shelf life: limited shelf life, expiry date on the label
• technique(s): microbe id | staining: suitable
• suitability: suitable for microscopy, spore forming microorganisms
• Quality Level: 100

Description

Application

Schaeffer & Fulton′s Spore Stain-Kit is used for staining bacterial spores

General description

In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the endospores by steaming the bacterial emulsion. Malachite green is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safranin. Endospores are green colored while bacteria show up as red bodies.

Other Notes

Kit contents:
Schaeffer & Fulton`s Spore Stain A: Malachite green 50 g/l in water,
Schaeffer & Fulton`s Spore Stain B: Safarin O 5 g/l in water

Safety Information

• pictograms: GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H318,H361fd,H411
• pcodes: P202 - P273 - P280 - P305 + P351 + P338 - P308 + P313 - P391
• Hazard Classifications: Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2
• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable