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05038

Millipore

Agar

suitable for microbiology

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Synonym(s):
Agar-agar, Gum agar
Linear Formula:
(C12H18O9)n
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106212
NACRES:
NA.74

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

ign. residue

≤1.5%

loss

≤10% loss on drying

pH

5.5-7.5 (25 °C)

mp

≥85 °C

transition temp

gel point 32-35 °C

gel strength

>900 g/cm2

solubility

H2O: 1.5%, clear to almost clear (100 °C/15 min.)

cation traces

Ca: ≤2500 mg/kg
Fe: ≤100 mg/kg
Mg: ≤900 mg/kg
Pb: ≤5 mg/kg

application(s)

agriculture
microbiology

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Agar has been used as reagent for the following studies:
  • Plant cell culture studies.
  • As medium supplement for the growth of wild-type Arabidopsis thaliana (ecotype Columbia) and transgenic seeds.
  • For the solidification of culture medium of cork oak somatic embryos.
  • It may be used for incubation of single antibody-forming cell to investigate the plaque formation.
  • Agar suspension culture may be used for the analysis of cell transformation of palyoma virus.
Agar is a complex gelatinous carbohydrate, and is added to the LB broth (Lysogeny broth, Luria broth, Lennox broth, or Luria-Bertani medium), in order to form gel for bacteria to grow upon. LB Broth is the most widely used nutritionally rich medium, for the culture and growth of bacteria. There are several different formulations of Lb broth, but the composition is generally the same comprising of peptides and casein peptones, trace elements, mineral and vitamins.
This agar is recommended for gelling the microbiological culture media where a great transparence and brightness is required. Especially useful in immuno-electrophoretic procedures, nutritional studies (Vitamin Assay Media) or sensitivity testing procedures, where high purity and good diffusion of substances is essential. It is essentially free of impurities.

wgk_germany

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Developmentally and stress-induced small heat shock proteins in cork oak somatic embryos.
Puigderrajols P1, Jofre A, Mir G, Pla M, Verdaguer D, Huguet G, Molinas M
Journal of Experimental Botany, 53 (373), 1445-1452 (2002)
Hijacking membrane transporters for arsenic phytoextraction.
Melissa S LeBlanc et. Al.
Journal of Biotechnology, 163 (1), 1-9 (2013)
AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS.
MACPHERSON I, MONTAGNIER L
Virology, 291-294 (1964)
Agar and agarose biotechnological applications.
Armisen R.
Hydrobiologia, 221 (1), 157-166 (1991)
The structure of agar: Part I. Fractionation of a complex mixture of polysaccharides.
Duckworth M and Yaphe W.
Carbohydrate Research, 16 (1), 189-197 (1971)

Articles

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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